Downloadable Documents:
> Technical Data Sheet
> Western blotting Protocol
> Immunocytochemistry Protocol


Images:

Figure 1. MS701 is reactive against human, bovine, mouse, and rat samples. Shown is a Western blot image using HepG2 cells, with anti-Complex II 30kDa subunit labeled as a control.


Figure 2. HDFn cells labeled with MS701 as the primary antibody with a goat anti-mouse IgG1 secondary antibody conjugated to Alexa Fluor® 594.


Proteins > Nicotinamide nucleotide transhydrogenase (NNT) > NNT monoclonal antibody

NNT monoclonal antibody

Catalog No. MS701

$325.00 - 100 µg

UniProt Number: Q13423
Alternate Names: NAD(P) transhydrogenase, pyridine nucleotide transhydrogenase
Structure and Function: NNT is a mitochondrial inner membrane protein catalyzing the interconversion of NADH and NADPH within the matrix space of the organelle. It couples this reaction to proton translocation across the inner membrane. In the presence of an electrochemical proton gradient the production of NADPH is strongly favored. NADPH is required in the mitochondrial matrix to keep the ratio GSH:GSSG high via the NADPH dependent glutathione reductase. Thus NNT has an important role to play in defences against oxidative stress produced by the organelle.

A novel, unexpected role of NNT is in insulin secretion. The absence of NNT prevents glucose from closing the K+ATP channel and from opening the Ca++ channel in pancreatic beta cells. These defective beta cells also show a profound increase in ROS.
Disease Associations: n/a

Product Specifications
  
Applications: Western blotting and Immunocytochemistry
Species Reactivity: human, bovine, mouse, rat
Host Species: mouse
Isotype: IgG1, k
Clone ID: 8B4BB10
Immunogen: Human heart mitochondria
Concentration: 1 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 0.5 µg/mL for Western blotting, 5 µg/mL for Immunocytochemistry
Storage Conditions: 4°C
Country of Origin: USA
Citations:  


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