Nicotinamide nucleotide transhydrogenase (NNT) monoclonal antibody
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Catalog No. MS701
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$325.00 - 100 µg
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, In-Cell ELISA, Flow Cytometry
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Species Reactivity:
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human, bovine, mouse, rat
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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8B4BB10
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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0.5 µg/mL for Western blotting
5 µg/mL for Immunocytochemistry
4 µg/mL for In-Cell ELISA (0.4 µg/well)
1 µg/mL for Flow Cytometry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
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Figure 1. MS701 is reactive against human, bovine, mouse, and rat samples. Shown is a Western blot image using HepG2 cells, with anti-Complex II 30kDa subunit labeled as a control.
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ICC Images
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Figure 2. Mitochondrial localization of NNT in cultured, normal human skin fibroblasts (strain HDFn) labeled with MS701 as the primary antibody and an AlexaFluor® 594 goat anti-mouse IgG1 secondary antibody.
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IHC Images
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Figure 3. NNT immunohistochemistry in human cerebellum visualized with mAb MS701. NNT immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Image kindly provided by Nichola Lax and Doug Turnbull, Mitochondrial Research Group, Newcastle University.
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IP Images
(click to enlarge)
Figure 4. Nicotinamide nucleotide transhydrogenase (NNT) immunocaptured from human liver mitochondria, bovine heart mitochondria, rat liver mitochondria, mouse liver mitochondria, and HepG2 cells using MS701 anti-NNT monoclonal antibody.
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Flow Images
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Figure 5. HL-60 cells were stained with 1 µg/mL NNT antibody (MS701) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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