$475.00 (96 tests)
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Brief Description & Uses
The MitoProfile® Biogenesis Microplate Assay Kit
is used to determine whether mitochondrial biogenesis has been
up-regulated or down-regulated as a result of drug interactions or
conditions such as oxidative stress and mitochondrial genetic disease.
MS641 is particularly suited for high-throughput screening of
mitochondrial toxicity from antibiotic and antiviral drugs.
Compounds such as resveratrol and regimens such as caloric restriction
have also been demonstrated to increase
mitochondrial biogenesis in animal studies, and MS641 is suitable for
studies of any such up-regulating factors in human cells or patient
The principle of the assay is to compare the levels of Complex IV
(cytochrome c oxidase), a mitochondrial enzyme which has 3 subunits
encoded by mitochondrial DNA (mtDNA) and made by mitochondrial
ribosomes, with that of frataxin, a mitochondrial protein encoded by
nuclear DNA and made by cytosolic ribosomes.
here for a detailed overview of the biogenesis
Also available is a POC version:
MitoProfile® Biogenesis Dipstick Assay Kit
Biogenesis Microplate Assay Kit
For measuring inhibition of
mitochondrial biogenesis in cultured cells or in human blood and tissue
Store the AP reagent at -20°C. When
planning multiple experiments, aliquot AP reagent to prevent
Store all other components at 4°C.
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