MS620 MitoProfile Mitochondria Membrane Integrity Kit


$525.00 (255 g)

Please contact us for volume discounts.


Brief Description & Uses

Contains 5 mAbs against representative proteins for each of the primary structures of the mitochondrion:

• Outer Membrane - Porin (MSA03)
• Intermembrane Space - Cytochrome c (MSA06)
• Inner Membrane - Complex Vα (MS502) and Complex III Core 1 (MS303)
• Matrix Space - Cyclophilin D (MSA04)

This kit is useful in Western blotting to evaluate the structural integrity of isolated mitochondria. This kit is reactive against 39kDa human porin, 12kDa cytochrome c, 55kDa Complex Vα, 49kDa Complex III Core 1, and 21kDa Cyclophilin D. Human heart mitochondria are provided as standard control - mitochondria from other species and tissues are available upon request.


Specifications

Product: MitoProfile Mitochondria Membrane Integrity Kit

Kit Components:

Premixed cocktail of 255 g containing 5 monoclonal antibodies (see Table below), HHM control.

Catalog #: MS620
Lot #:
Host Species: Mouse
Concentration: 2.37 mg/ml in HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: The antibody cocktail (2.37mg/ml) should be diluted to a final working concentration of 10.2 g/ml for Western blotting
Applications: Western blotting detection of a protein from each mitochondrial compartment. Can be used to assess mitochondrial membrane integrity during and after purification.
Antigen Species Cross-Reactivity: human, bovine, mouse, rat
Storage: 4C
Country of Origin: USA



Product Components

ANTIGEN CAT # CLONE ID AMOUNT
(g)
FINAL CONC.
(g/ml)
IMMUNOGEN
Porin MSA03 20B12AF2 50 2 Human VDAC1
Cyclophilin D MSA04 E11AE12BD4 50 2 Rat CypD/PPIF
C-III-Core 1 MS303 16D10AD9AH5 50 2 Bovine Heart Complex II,III
Cytochrome C MSA06 37BA11 5 0.1 Human Cytochrom C
C-V-α MS502 7H10BD4F9 100 4 Bovine Complex V



Product References

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References for Mitochondrial Membrane Integrity

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Images



Downloadable Information
MS620 Specifications Western Blotting Protocol


Additional Information
Product Components
Product Images
Product References
Protein References


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