MS408 Complex IV subunit IV
monoclonal antibody (alternate to 407)


$295.00 (100 g)

Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.



Brief Description & Uses

100 g of monoclonal antibody reactive against the 18 kDa COX (Complex IV) subunit IV. MS408 also labels COX IV in immunocytochemical applications. Reacts only with human and bovine, but better than MS407 for immunocytochemistry in these species. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

Variations in the synthesis of cytochrome c oxidase subunits from the two genomes (mitochondrial and nuclear) have been described as markers for several cancers including those of the prostate, and thyroid. Additionally this ratio has been reported to identify areas of the brain damaged in Alzheimer’s disease and also to relate to the overall regulation of mitochondrial biogenesis in exercise and diet. To examine this ratio in immunocytochemistry we offer two mAbs against mitochondrially encoded subunits I and II (MS404 and MS405, respectively) and mAbs against two nuclear encoded subunits IV and Va (MS408 and MS409, respectively). The latter two are retained in mitochondria exhibiting heterolpasmy or mosaiscism of altered mtDNA transcription e.g. in anti-retroviral therapy. Therefore an immunocytochemical study using a combination of subunits I or II alongside IV or Va could reveal changes in the levels of mtDNA or mtDNA encoded proteins.


Specifications

Product: Complex IV subunit IV
monoclonal antibody (alternative to MS407)
Catalog #: MS408
Lot #:
Clone ID: 10G8D12C12
Host Species and Isotype: Mouse IgG2a, κ
Immunogen: Bovine Complex IV subunit IV
Concentration: 1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 1 g/ml for Western blotting, 5 g/ml for Immunocytochemistry
Applications: Western blotting, Immunocytochemistry
(heat-induced antigen-retrieval improves signal)
Antigen Species Cross-Reactivity: human and bovine
Storage: 4C
Country of Origin: USA



Product References

Genome Res. 2007 Aug;17:1319-1326.
"Direct linkage of mitochondrial genome variation to risk factors for type 2 diabetes in conplastic strains."


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References for Complex IV subunit IV

There are no references currently posted for this product.




Images

Complex IV subunit IV Immunocytochemistry Image


Downloadable Information
MS101 Specifications Western Blotting Protocol Immunocytochemistry Protocol Research Playbook


Additional Information
Product Images
Product References
Protein References


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Secondary Antibodies
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