MitoSciences: MSF31 Frataxin Protein Quantity Dipstick Assay Kit

Enzymes > Frataxin > Frataxin Protein Quantity Dipstick Assay Kit

Frataxin Protein Quantity Dipstick Assay Kit

Catalog No. MSF31

$325.00 - 30 tests
$595.00 - 90 tests

Product Description

Contains 30 or 90 dipsticks and necessary components to assess the levels of frataxin. The kit includes sufficient materials to evaluate several unknown samples and to determine the linear working range of a normal sample.

Learn more about Dipstick Protein Quantity Assays >

Specifications

Data

Documentation

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Product Specifications
Species Reactivity:	human
Storage Conditions:	Store dipsticks and gold-conjugated secondary antibody at room temperature out of direct sunlight in their provided containers. Store buffers at 4° C.
Country of Origin:	USA

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Specifications

Data

Documentation

Citations

Figure 1

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Figure 1. An example using MSF31 to quantify frataxin levels from β -lymphocyte cell culture samples derived from control individuals and Friedreich's Ataxia (FA) patients.

Shown is a 1:2 dilution series using a positive control sample. Approximately 6 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 4 µg of control sample. A one-site hyperbola line was generated for best-fit analysis using GraphPad.

Figure 2

(click to enlarge)
Figure 2. An example using MSF31 to quantify frataxin levels from β -lymphocyte cell culture samples derived from control individuals and Friedreich's Ataxia (FA) patients.

Based on the above standard curve values, of protein extract for the controls and FA patient samples. (Note: for a statistical analysis, it is preferred to use two dipsticks for each sample; intra-assay CV's are typically <=10%.) For this analysis, the FA patients had between 550 - 925 GAA repeats on the smaller allele. Using GraphPad software, the signal intensity from the standard curve was interpolated and the relative amount of frataxin in the patients, as compared to the controls, was determined. Based on the above analysis, the patient samples had between 13% and 20% of frataxin levels compared to the control.

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