Build Your Own Microplate ELISA

Choose From Among 30 Metabolic Enzymes Representing Over a Dozen Different Metabolic Pathways

FlexPlex™ is a revolutionary yet incredibly simple system for quantitatively and simultaneously measuring the levels of multiple metabolic enzymes in a tissue or cell extract. Using tried-and-true sandwich ELISA technology, in which capture/detector antibody pairs are used to first immobilize and then measure a protein, the FlexPlex™ system merely extends this concept by allowing for the parallel analysis of up to 12 different proteins on a single microplate. Learn more about sandwich ELISA technology.

Each FlexPlex™ Module includes 4 individual microplate strips, all of which are pre-coated with a monoclonal capture antibody against a single target, plus a vial of detector antibody. The FlexPlex™ Starter Pack includes a microplate frame plus all necessary buffers and other reagents to perform a complete plate's worth of experiments. FlexPlex™ Modules can be configured in any combination desired by the investigator, whether it be a single strip for each of 12 different targets, or a complete plate for 1 target.

Benefits of the FlexPlex™ System:

• Specificity - Each Module is tested in multiple sample types, including patient-derived cell lines wherever possible. Plus, all capture antibodies (and over 90% of detectors) are monoclonal, and their targets are characterized by mass spec (along with WB and ICC whenever possible) during development.
Learn more about MitoSciences' ELISA mAb development process.

• Coverage - Multiple enzymes can be measured in parallel, and the available Modules include key nodes from over a dozen different metabolic pathways and processes. For the majority of the FlexPlex™ targets this is the first time that an ELISA assay has been made available by any vendor.

• Sensitivity - Analysis can typically be conducted with as little as 2 µg of sample per well.

• Cost-Effectiveness - Analysis can be conducted one Module at a time, or even one strip at a time, significantly reducing costs.

• Simplicity - The FlexPlex™ protocol requires approximately 1 hour of total hands-on time per complete plate of assays.

• Accessibility - Compatible with virtually all colorimetric plate readers.

• Extensibility - Immunocapture conditions have been carefully developed using proprietary systems in order to maintain multi-subunit enzymes in their completely intact, functionally active states, allowing for follow-up analysis of specific activity and post-translational modifications.

Sample Data Set
Shown below are the results from a broad screen of the expression of 27 metabolic enzymes representing 8 different metabolic functions, which was performed using the FlexPlex™ system on human heart homogenate and HepG2 cells. Generating this data set required only 1 day, with just 3 hours of total hands-on time. Measuring several additional samples simultaneously adds only minimal additional effort, and provides multifold expansion of the data set.

Figure 1. Differences in expression of 27 metabolic enzymes in human heart homogenate (HHH) versus human liver cells (HepG2) as measured by FlexPlex™ Modules. Detergent extracts from HepG2 cells and HHH cells were prepared. Each sample was simultaneously analyzed by the 27 FlexPlex™ Modules shown. Within the working range of each assay, the same amount of protein was compared (in most cases 10 µg), and the log of the determined signal ratio is represented. No difference between the samples is represented as 0.00, while increased expression is a positive log function and decreased expression negative. Not surprisingly, OXPHOS and fatty acid oxidation enzymes are generally expressed more highly in HHH than in HepG2 cells, which being cancer cells tend to favor glycolysis for energy production.

Ordering Information

Step 1 of 2: Order Your FlexPlex™ Starter Pack(s)

Order 1 Starter Pack for every 12 strips (1 complete microplate) that you plan to run.

Each Starter Pack includes:
• 1X Extraction buffer
• 20X Wash buffer
• 10X Blocking buffer
• 1X HRP development solution
• (1) microplate frame

FlexPlex™ Starter Pack - $50 each
Introductory Offer: For a limited time get one free Starter Pack for every 3 Modules purchased!

Step 2 of 2: Choose Your FlexPlex™ Modules

Each Module includes:
• (4) Pre-coated microplate strips
• 10X Detector antibody
• 10X HRP label

Available FlexPlex™ Modules
Cat. No. Enzyme Metabolic Pathway Price Qty  
MSFX-26 2,4 dienoyl-CoA reductase (DECR1) Fatty Acid Oxidation (Mitochondrial) $125.00
MSFX-21 Acetyl-CoA acyltransferase, mitochondrial (ACAA2) Fatty Acid Oxidation (Mitochondrial) $125.00
MSFX-18 Apoptosis-inducing factor (AIF) Apoptosis $125.00
MSFX-23 Bile acid-CoA amino acid N-acyltransferase (BAAT) Fatty Acid Oxidation (Peroxisomal) $125.00
MSFX-25 Carbamoyl-phosphate synthase I (CPS1) Urea Cycle $125.00
MSFX-24 Catalase (CAT) Amino Acid Deamination
Fatty Acid Oxidation (Peroxisomal)
MSFX-1 Complex I Oxidative Phosphorylation $125.00
MSFX-2 Complex II Oxidative Phosphorylation $125.00
MSFX-3 Complex III Oxidative Phosphorylation $125.00
MSFX-4 Complex IV Oxidative Phosphorylation $125.00
MSFX-5 Complex V (ATP synthase) Oxidative Phosphorylation $125.00
MSFX-16 Cytochrome c (CYTC) Apoptosis $125.00
MSFX-22 Delta-1-pyrroline-5-carboxylate dehydrogenase (ALDH4A1) Xenobiotic Metabolism $125.00
MSFX-13 Dicarbonyl/L-xylulose reductase (DCXR) Uronate cycle $125.00
MSFX-27 Epoxide hydrolase 1 (EPHX1) Xenobiotic Metabolism $125.00
MSFX-7 Frataxin (FXN)   $125.00
MSFX-14 Fumarate hydrase (FH) Citric Acid Cycle $125.00
MSFX-6 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) Glycolysis $125.00
MSFX-9 Medium-chain acyl CoA dehydrogenase (MCAD) Fatty Acid Oxidation (Mitochondrial) $125.00
MSFX-17 Mitofilin (MF)   $125.00
MSFX-20 Monoamine oxidase A (MAOA) Xenobiotic Metabolism $125.00
MSFX-19 Monoamine oxidase B (MAOB) Xenobiotic Metabolism $125.00
MSXF-8 Nicotinamide nucleotide transhydrogenase (NNT) Oxidative Phosphorylation $125.00
MSFX-29 Peroxisomal multifunctional enzyme 1 (MFE1) Fatty Acid Oxidation (Perxisomal) $125.00
MSFX-15 Pyruvate dehydrogenase (PDH) Cictric Acid Cycle
MSFX-28 Ribonuclease UK114 (HRSP12)   $125.00
MSFX-10 Short-chain 3-hodroxyacyl acyl-CoA dehydrogenase (SCHAD) Fatty Acid Oxidation (Mitochondrial) $125.00
MSFX-30 Sirtuin 1 (SIRT1)   $125.00
MSFX-12 Superoxide dismutase (SOD2) Free Radical Scavenging $125.00
MSFX-11 Trifunctional protein, mitochondrial (TFP) Fatty Acid Oxidation (Mitochondrial) $125.00

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