CARTFlexPlex™ is a revolutionary yet incredibly simple system for quantitatively and simultaneously measuring the levels of multiple metabolic enzymes in a tissue or cell extract. Using tried-and-true sandwich ELISA technology, in which capture/detector antibody pairs are used to first immobilize and then measure a protein, the FlexPlex™ system merely extends this concept by allowing for the parallel analysis of up to 12 different proteins on a single microplate. Learn more about sandwich ELISA technology.
Each FlexPlex™ Module includes 4 individual microplate strips, all of which are pre-coated with a monoclonal capture antibody against a single target, plus a vial of detector antibody. The FlexPlex™ Starter Pack includes a microplate frame plus all necessary buffers and other reagents to perform a complete plate's worth of experiments. FlexPlex™ Modules can be configured in any combination desired by the investigator, whether it be a single strip for each of 12 different targets, or a complete plate for 1 target.
Benefits of the FlexPlex™ System:
• Specificity - Each Module is tested in multiple sample types, including patient-derived cell lines wherever possible. Plus, all capture antibodies (and over 90% of detectors) are monoclonal, and their targets are characterized by mass spec (along with WB and ICC whenever possible) during development.
Learn more about MitoSciences' ELISA mAb development process.
• Coverage - Multiple enzymes can be measured in parallel, and the available Modules include key nodes from over a dozen different metabolic pathways and processes. For the majority of the FlexPlex™ targets this is the first time that an ELISA assay has been made available by any vendor.
• Sensitivity - Analysis can typically be conducted with as little as 2 µg of sample per well.
• Cost-Effectiveness - Analysis can be conducted one Module at a time, or even one strip at a time, significantly reducing costs.
• Simplicity - The FlexPlex™ protocol requires approximately 1 hour of total hands-on time per complete plate of assays.
• Accessibility - Compatible with virtually all colorimetric plate readers.
• Extensibility - Immunocapture conditions have been carefully developed using proprietary systems in order to maintain multi-subunit enzymes in their completely intact, functionally active states, allowing for follow-up analysis of specific activity and post-translational modifications.
Sample Data Set
Shown below are the results from a broad screen of the expression of 27 metabolic enzymes representing 8 different metabolic functions, which was performed using the FlexPlex™ system on human heart homogenate and HepG2 cells. Generating this data set required only 1 day, with just 3 hours of total hands-on time. Measuring several additional samples simultaneously adds only minimal additional effort, and provides multifold expansion of the data set.
