Protein Quantity Sandwich ELISAs (FlexPlex™)


Sandwich ELISAs provide high-throughput quantitative measurements of protein levels in cells and tissues. Sandwich ELISAs are a well-accepted technology in research. In this new era of systems biology, investigators want to measure the levels of several proteins simultaneously. MitoSciences provides sandwich ELISA assays in "modules" termed FlexPlex™. Users can customize their assay by selecting 3 modules from our selection of dozens of analytes. Each of the 3 modules in an assay contains enough wells and reagents for 32 measurements. The 3 modules can be for the same target, or 3 different targets.

As an example, a customized FlexPlex™ Assay has been designed to quantify the amounts of the OXPHOS complexes in tissue and cultured cells. Background information, methods, results and discussion are all described in our FlexPlex™ Application Note.



Ordering Information
The Starter Pack includes:

• 1X Extraction buffer
• 20X Wash buffer
• 10X Blocking buffer
• 1X HRP development solution
• (1) microplate frame
MitoSciences Sandwich ELISA FlexPlex starter pack
Each Module includes:

• (4) Pre-coated microplate strips
• 10X Detector antibody
• 10X HRP label
MitoSciences Sandwich ELISA FlexPlex module


To build your own microplate ELISA, choose up to 3 modules from the drop-down menus. The 3 modules can be for the same target, or 3 different targets. Once your modules have been selected, click the Add To Cart button below. The Starter Pack will be added automatically.

                 

Introductory Offer: For a limited time get one free Starter Pack for every 3 Modules purchased!
$375 - 1 Full Plate (Regular $425)

Regular Price is $125 per Module + $50 for the Starter Pack

Available FlexPlex™ Modules
Cat. No. Target Price/Module (4 Strips)
MSFX-26 2,4 dienoyl-CoA reductase (DECR1) $125.00  Full Plate >
 Add to Plate >
MSFX-22 Acetyl-CoA acyltransferase, mitochondrial (ACAA2) $125.00  Full Plate >
 Add to Plate >
MSFX-40 Aconitase (ACO2) $125.00  Full Plate >
 Add to Plate >
MSFX-41 Alpha-ketoglutarate dehydrogenase (OGDH) $125.00  Full Plate >
 Add to Plate >
MSFX-18 Apoptosis-inducing factor (AIF) $125.00  Full Plate >
 Add to Plate >
MSFX-23 Bile acid-CoA amino acid N-acyltransferase (BAAT) $125.00  Full Plate >
 Add to Plate >
MSFX-25 Carbamoyl-phosphate synthase I (CPS1) $125.00  Full Plate >
 Add to Plate >
MSFX-35 Carboxylesterase 1 (CES1) $125.00  Full Plate >
 Add to Plate >
MSFX-31 Carnitine palmitoyltransferase 2 (CPT2) $125.00  Full Plate >
 Add to Plate >
MSFX-24 Catalase (CAT) $125.00  Full Plate >
 Add to Plate >
MSFX-1 Complex I $125.00  Full Plate >
 Add to Plate >
MSFX-2 Complex II $125.00  Full Plate >
 Add to Plate >
MSFX-3 Complex III $125.00  Full Plate >
 Add to Plate >
MSFX-4 Complex IV $125.00  Full Plate >
 Add to Plate >
MSFX-5 Complex V (ATP synthase) $125.00  Full Plate >
 Add to Plate >
MSFX-43 Creatine Kinase M (CKM) $125.00  Full Plate >
 Add to Plate >
MSFX-16 Cytochrome c (CYTC) $125.00  Full Plate >
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MSFX-22 Delta-1-pyrroline-5-carboxylate dehydrogenase (ALDH4A1) $125.00  Full Plate >
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MSFX-13 Dicarbonyl/L-xylulose reductase (DCXR) $125.00  Full Plate >
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MSFX-34 Electron transfer flavoprotein (ETF) subunit alpha (ETFA) $125.00  Full Plate >
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MSFX-27 Epoxide hydrolase 1 (EPHX1) $125.00  Full Plate >
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MSFX-7 Frataxin (FXN) $125.00  Full Plate >
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MSFX-14 Fumarate hydrase (FH) $125.00  Full Plate >
 Add to Plate >
MSFX-44 Glucose-6-phosphate isomerase (G6PI) $125.00  Full Plate >
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MSFX-6 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) $125.00  Full Plate >
 Add to Plate >
MSFX-9 Medium-chain acyl CoA dehydrogenase (MCAD) $125.00  Full Plate >
 Add to Plate >
MSFX-17 Mitofilin (MF) $125.00  Full Plate >
 Add to Plate >
MSFX-20 Monoamine oxidase A (MAOA) $125.00  Full Plate >
 Add to Plate >
MSFX-19 Monoamine oxidase B (MAOB) $125.00  Full Plate >
 Add to Plate >
MSXF-8 Nicotinamide nucleotide transhydrogenase (NNT) $125.00  Full Plate >
 Add to Plate >
MSFX-29 Peroxisomal multifunctional enzyme (ECHD) $125.00  Full Plate >
 Add to Plate >
MSFX-33 Pyruvate carboxylase, mitochondrial (PC) $125.00  Full Plate >
 Add to Plate >
MSFX-15 Pyruvate dehydrogenase (PDH) $125.00  Full Plate >
 Add to Plate >
MSFX-28 Ribonuclease UK114 (HRSP12) $125.00  Full Plate >
 Add to Plate >
MSFX-10 Short-chain 3-hodroxyacyl acyl-CoA dehydrogenase (SCHAD) $125.00  Full Plate >
 Add to Plate >
MSFX-30 Sirtuin 1 (SIRT1) $125.00  Full Plate >
 Add to Plate >
MSFX-12 Superoxide dismutase (SOD2) $125.00  Full Plate >
 Add to Plate >
MSFX-11 Trifunctional protein, mitochondrial (TFP) subunit alpha and beta (HADHA / HADHB) $125.00  Full Plate >
 Add to Plate >


Sample Data Set
Shown below are the results from a broad screen of the expression of 27 metabolic enzymes representing 8 different metabolic functions, which was performed using the FlexPlex™ system on human heart homogenate and HepG2 cells. Generating this data set required only 1 day, with just 3 hours of total hands-on time. Measuring several additional samples simultaneously adds only minimal additional effort, and provides multifold expansion of the data set.


Figure 1. Differences in expression of 27 metabolic enzymes in human heart homogenate (HHH) versus human liver cells (HepG2) as measured by FlexPlex™ Modules. Detergent extracts from HepG2 cells and HHH cells were prepared. Each sample was simultaneously analyzed by the 27 FlexPlex™ Modules shown. Within the working range of each assay, the same amount of protein was compared (in most cases 10 µg), and the log of the determined signal ratio is represented. No difference between the samples is represented as 0.00, while increased expression is a positive log function and decreased expression negative. Not surprisingly, OXPHOS and fatty acid oxidation enzymes are generally expressed more highly in HHH than in HepG2 cells, which are cancer cells and utilize glycolysis, wheras normal heart relies on oxidative phosphorylation for energy production.
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Application Note
Sandwich ELISA Application Note

Sandwich ELISA Application Note
Review examples of data generated by MitoSciences using our FlexPlex™ modular sandwich ELISA platform.





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