MitoSciences: MS861 Cell Fractionation Kit

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Cell Fractionation Kit Standard

Catalog No. MS861

$195.00 - 1 Kit

Product Description

The Cell Fractionation Kit allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear fractions from cultured cells. Unlike competing products, this kit does not require mechanical disruption of the sample. This is an important performance characteristic, as mechanical disruption can also disrupt mitochondrial membranes, causing the biologically-irrelevant release of proteins. Particularly useful in studying apoptosis and the movement of pro-apoptotic proteins such as cytochrome c.

A companion product is also available which allows for the fractionation of cells grown in 96-well plates: Cell Fractionation Kit HT (MS862).

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Product Specifications
Kit Components:	350mL of Buffer A (2 x 175 mL aliquots) 25 µL of Detergent I 1 mL of Detergent II 10 mL of 5X SDS Sample Buffer.
Storage Conditions:	Store Buffer A, 5X SDS Sample Buffer and Detergent II at -20°C. Store Detergent I at -80°C.
Country of Origin:	USA

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Specifications

Data

Documentation

Citations

Figure 1

(click to enlarge)
Figure 1. In this experiment, apoptosis was induced in Jurkat and 143B osteosarcoma cells by FAS and also by treatment with staurosporine (HeLa cells were also treated, but only with STS). Mitochondrial and cytoplasmic fractions were isolated (using kit Cell Fractionation Kit MS861) and probed using MSA12.

As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.

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