Very long-chain acyl-CoA dehydrogenase (VLCAD) monoclonal antibody
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Catalog No. MS707
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$325.00 - 100 µg
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This antibody can also be purchased as part of a Sample Pack.
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Product Specifications
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Applications:
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Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
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Species Reactivity:
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human, mouse, rat, bovine
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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6A9AF2
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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5 µg/mL for Immunocytochemistry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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ICC Images
(click to enlarge)
Figure 1. Immunocytochemistry image of MS707 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (6A9AF2, 5 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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IP Images
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Figure 2. Very-long-chain acyl CoA dehydrogenase (VLCAD) immunocaptured from human liver mitochondria using MS707 anti-VLCAD monoclonal antibody.
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IHC Images
(click to enlarge)
Figure 3. VLCAD immunohistochemistry in human cerebellum visualized with mAb MS707. VLCAD immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
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