home
- Mito Sciences:
  Start here to find the products and information you need.
news & updates
company
contact
products
place order
links

Western Blotting
Products
Immunocytochemistry Products
Immunocapture
Products

Western Blotting Products

Mitosciences provides either individual antibodies or premixed cocktails for the detection of mitochondrial proteins by Western blotting. These antibodies bind various subunits of OXPHOS complexes I, II, III, IV and V in addition to other proteins of the inner mitochondrial membrane. A series of antibodies are also available against subunits of the pyruvate dehydrogenase complex (PDH).

MS102-200 Complex-I Subunit Western Blotting Kit
$375.00
Kit MS102 contains a total of 200µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex I subunits NDUFA9, NDUFS3, 20 kDa (likely ND6) along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex I purity or to conveniently determine the levels of 3 key Complex I subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS111, MS110, MS105 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together

Click to download the specifications PDF

MS102-400 Complex-I Subunit Western Blotting Kit
$695.00
Kit MS102 contains a total of 400µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex I subunits NDUFA9, NDUFS3, 20 kDa (likely ND6) along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex I purity or to conveniently determine the levels of 3 key Complex I subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS111, MS110, MS105 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS103 Western Blotting Detection of GRIM-19
$250.00
100µg of monoclonal antibody reactive against the 19 kDa Complex I (NADH dehydrogenase) subunit GRIM-19. Human heart mitochondria are also included as a Western blotting control. Interestingly GRIM-19 is also present in the nucleus and cytoplasm. Its expression is necessary for interferonβ/retinoic acid induced tumor mortality.

MS104 Western Blotting Detection of NDUFS4
$250.00
100µg of monoclonal antibody reactive against the 22 kDa Complex I (NADH dehydrogenase) subunit NDUFS4. Human heart mitochondria are also included as a Western blotting control.

MS105 Western blotting Detection of Complex I 20 kDa (likely ND6)
$250.00
100µg of monoclonal antibody reactive against a Complex I (NADH dehydrogenase) 20 kDa subunit. Preliminary data (2D blotting and Rho0 blotting) indicate this antibody is reactive against the mtDNA encoded ND6 subunit. Human heart mitochondria are also included as a Western blotting control.

MS107 Western blotting Detection of Complex I 15 kDa (likely NDUFA6)
$250.00
100µg of monoclonal antibody reactive against a 15 kDa Complex I (NADH dehydrogenase) subunit. Preliminary data(2D blotting) indicate this subunit is the NDUFA6 subunit. Human heart mitochondria are also included as a Western blotting control.

MS108 Western blotting Detection of NDUFB6
$250.00
100µg of monoclonal antibody reactive against the 20 kDa Complex I (NADH dehydrogenase) subunit NDUFB6. Human heart mitochondria are also included as a Western blotting control.

MS109 Western blotting Detection of Complex I 8 kDa
$250.00
100µg of monoclonal antibody reactive against an 8 kDa Complex I (NADH dehydrogenase) subunit. Human heart mitochondria are also included as a Western blotting control.

MS110 Western blotting Detection of NDUFS3
$250.00
100µg of monoclonal antibody reactive against the 30 kDa Complex I (NADH dehydrogenase) subunit NDUFS3. Human heart mitochondria are also included as a Western blotting control.

MS111 Western blotting Detection of NDUFA9
$250.00
100µg of monoclonal antibody reactive against the 39 kDa Complex I (NADH dehydrogenase) subunit NDUFA9. Human heart mitochondria are also included as a Western blotting control.

MS112 Western blotting Detection of NDUFS3
$250.00
100µg of monoclonal antibody reactive against the 30 kDa Complex I (NADH dehydrogenase) subunit NDUFS3 in humans and C.elegans. Human heart mitochondria are also included as a Western blotting control.

MS202-300 Complex-II Subunit Western Blotting Kit
$375.00
Kit MS202 contains a total of 300µg of antibodies and is a premixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 3 mAbs (MS204, MS203 and MS502, respectively) can be bought seperately at a 10% discount when all three are ordered together

MS202-600 Complex-II Subunit Western Blotting Kit
$695.00
Kit MS202 contains a total of 600µg of antibodies and is a premixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 3 mAbs (MS204, MS203 and MS502, respectively) can be bought seperately at a 10% discount when all three are ordered together.

MS203 Western blotting detection of Complex II 30 kDa Ip
$250.00
100µg of monoclonal antibody reactive against the Complex II 30 kDa Ip subunit in Western blotting applications.

MS204 Western blotting detection of Complex II 70 kDa Fp
$250.00
100µg of monoclonal antibody reactive against the Complex II 70 kDa Fp subunit in Western blotting applications.

MS302-200 Complex-III Subunit Western Blotting Kit
$375.00
Kit MS302 contains a total of 200µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex III subunits Core1, 25 kDa Rieske FeS, 10 kDa subunit along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex I purity or to conveniently determine the levels of 3 key Complex III subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS303, MS305, MS306 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS302-400 Complex-III Subunit Western Blotting Kit
$695.00
Kit MS302 contains a total of 400µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex III subunits Core1, 25 kDa Rieske FeS, 10 kDa subunit along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex I purity or to conveniently determine the levels of 3 key Complex III subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS303, MS305, MS306 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS303 Western blotting detection of Complex III Core 1 protein
$250.00
100µg of monoclonal antibody reactive against the complex III protein core 1. Human heart mitochondria are also included as a Western blotting control.

MS304 Western blotting detection of Complex III Core 2 protein
$250.00
100µg of monoclonal antibody reactive against the complex III protein core 2. Human heart mitochondria are also included as a Western blotting control.

MS305 Western blotting detection of Complex III Rieske Protein
$250.00
100µg of monoclonal antibody reactive against the complex III Rieske subunit. Human heart mitochondria are also included as a Western blotting control.

MS306 Western blotting detection of Complex III 10 kDa protien
$250.00
100µg of monoclonal antibody reactive against a complex III 10 kDa subunit. Human heart mitochondria are also included as a Western blotting control.

MS402-300 Complex-IV Subunit Western Blotting Kit
$375.00
Kit MS402 contains a total of 300µg of antibodies and is a premixed solution of 4 monoclonal antibodies against COXI, COX IV, COX VIc along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex IV purity or to conveniently determine the levels of 3 key Complex IV subunits in patient samples relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS404, MS407, MS414 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS402-600 Complex-IV Subunit Western Blotting Kit
$695.00
Kit MS402 contains a total of 600µg of antibodies and is a premixed solution of 4 monoclonal antibodies against COXI, COX IV, COX VIc along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex IV purity or to conveniently determine the levels of 3 key Complex IV subunits in patient samples relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS404, MS407, MS414 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS403 Western Blotting Detection of SURF-1
$250.00
100µg of monoclonal antibody reactive against the complex IV assembly factor SURF-1. Cellular levels of SURF-1 are highest in growing cultured cells.

MS404 Western Blotting Detection of C-IV subunit I
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit I . Human heart mitochondria are also included as a Western blotting control.

MS405 Western blotting Detection of C-IV subunit II
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit II. Human heart mitochondria are also included as a Western blotting control.

MS406 Western Blotting Detection of C-IV subunit III
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit III. Human heart mitochondria are also included as a Western blotting control.

MS407 Western blotting Detection of C-IV subunit IV
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit IV . Human heart mitochondria are also included as a Western blotting control. MS408 also binds COX subunit IV.

MS408 (alternate to 407) Western Blotting Detection of C-IV subunit IV
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit IV . Human heart mitochondria are also included as a Western blotting control. MS407 also binds COX subunit IV.

MS409 Western Blotting Detection of C-IV subunit Va
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit Va. Human heart mitochondria are also included as a Western blotting control.

MS410 Western Blotting Detection of C-IV subunit Vb
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit Vb. Human heart mitochondria are also included as a Western blotting control.

MS411 Western Blotting Detection of C-IV subunit VIaH
$250.00
250µg of monoclonal antibody reactive against the COX (complex IV) subunit VIa and specific to the 'heart' isoform. Bovine heart mitochondria are also included as a Western blotting control.

MS412 Western Blotting Detection of C-IV subunit VIaL
$250.00
250µg of monoclonal antibody reactive against the COX (complex IV) subunit VIa and specific to the 'liver' isoform.

MS413 Western Blotting Detection of C-IV subunit VIb
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit VIb. Human heart mitochondria are also included as a Western blotting control.

MS414 Western Blotting Detection of C-IV subunit VIc
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit VIc. Human heart mitochondria are also included as a Western blotting control.

MS415 Western Blotting Detection of C-IV subunit VIIaH&VIIaL;
$250.00
250µg of monoclonal antibody reactive against the COX (complex IV) subunit VIIa and reacting against both 'liver' and 'heart' isoforms. Bovine heart mitochondria are also included as a Western blotting control.

MS416 Western Blotting Detection of C-IV subunit VIIb
$250.00
250µg of monoclonal antibody reactive against the COX (complex IV) subunit VIIb. Bovine heart mitochondria are also included as a Western blotting control.

MS502 Western Blotting Detection of C-Vα.
$250.00
100µg of monoclonal antibody reactive against complex V α subunit. Human heart mitochondria are also included as a Western blotting control.

MS503 Western Blotting Detection of C-Vβ
$250.00
100µg of monoclonal antibody reactive against complex V β subunit. Human heart mitochondria are also included as a Western blotting control.

MS504 Western Blotting Detection of C-V subunit d
$250.00
100µg of monoclonal antibody reactive against complex V d subunit. Human heart mitochondria are also included as a Western blotting control.

MS505 Western Blotting Detection of C-V OSCP subunit
$250.00
100µg of monoclonal antibody reactive against complex V OSCP subunit. Human heart mitochondria are also included as a Western blotting control.

MS506 Western Blotting Detection of C-V IF1 subunit
$250.00
100µg of monoclonal antibody reactive against complex V IF1 subunit. The antibody shows in Western blotting of whole mitochondria shows reactivity with two bands at 10 kDa and at 18 kDa, but in isolated F1F0 only the band at 10 kDa is observed (IF1). The 18 kDa band is the subunit IV of Complex IV. Human heart mitochondria are also included as a Western blotting control.

MS507 Western Blotting Detection of C-Vα. Broad cross-reactivity
$250.00
100µg of monoclonal antibody reactive against the F1F0 ATP synthase, Complex V α subunit. This mAb has broad species cross-reactivity including animal and plant subunits. Human heart mitochondria are also included as a Western blotting control.

MS508 Western Blotting Detection of C-V F6 subunit
$250.00
100µg of monoclonal antibody reactive against the F1F0 ATP synthase, Complex V F6 subunit. Human heart mitochondria are also included as a Western blotting control.

MS509-200 Complex V Subunit Western Blotting Kit
$375.00
Kit MS509 contains a total of 200µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex V subunits F1α, OSCP,F6 along with an antibody against Complex I NDUFA9 as a control. This cocktail can be used to verify isolated Complex V purity or to conveniently determine the levels of 3 key Complex V subunits in patient cell lines relative to the control protein (Complex I NDUFA9). Alternatively all 4 mAbs (MS502, MS505, MS508 and MS111, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS509-400 Complex V Subunit Western Blotting Kit
$695.00
Kit MS509 contains a total of 400µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex V subunits F1α, OSCP,F6 along with an antibody against Complex I NDUFA9 as a control. This cocktail can be used to verify isolated Complex V purity or to conveniently determine the levels of 3 key Complex V subunits in patient cell lines relative to the control protein (Complex I NDUFA9). Alternatively all 4 mAbs (MS502, MS505, MS508 and MS111, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS601-300 Total OXPHOS Complexes Detection Kit
$375.00
Kit MS601 contains a total of 300µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes. Human heart mitochondria are also included as a Western blotting control.

MS601-600 Total OXPHOS Complexes Detection Kit
$695.00
Kit MS601 contains a total of 600µg of antibodies and is apremixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes. Human heart mitochondria are also included as a Western blotting control.

MSA02 Western blotting Detection of ANT
$250.00
100µg of monoclonal antibody reactive against the adenine nucleotide translocase (ANT). Human heart mitochondria are also included as a Western blotting control.

MSA03 Western Blotting Detection of Porin
$250.00
100µg of monoclonal antibody reactive against human porin (VDAC1) for Western blotting applications. Human heart mitochondria are also included as a Western blotting control.

MSA04 Western blotting Detection of Cyclophilin D
$250.00
100µg of monoclonal antibody reactive against Cyclophilin 'D' subunit (P30405). Human heart mitochondria are also included as a Western blotting control.

MSP02 - 100 PDH Subunit Western Blotting Kit
$295.00
100µl of an antibody cocktail containing a total of 150µg of monoclonal antibodies reactive against PDH subunits. Human heart mitochondria are also included as a Western blotting control.

MSP02 - 250 PDH Subunit Western Blotting Kit
$645.00
250µl of an antibody cocktail containing a total of 375µg of monoclonal antibodies reactive against PDH subunits. Human heart mitochondria are also included as a Western blotting control.

MSP03 Western Blotting Detection of PDH-E1α.
$250.00
100µg of monoclonal antibody reactive against PDH subunit E1α Human heart mitochondria are also included as a Western blotting control.

MSP04 Western Blotting Detection of PDH - E1β
$250.00
100µg of monoclonal antibody reactive against PDH subunit E1β. Human heart mitochondria are also included as a Western blotting control.

MSP05 Western Blotting Detection of PDH-E2
$250.00
100µg of monoclonal antibody reactive against PDH subunit E2. Human heart mitochondria are also included as a Western blotting control.

MSP06 Western Blotting Detection of PDH-E2/E3bp
$250.00
100µg of monoclonal antibody reactive against the PDH subunit E2/E3bp. Also included are 50µg of human heart mitochondria to be used as Western blotting control. Preliminary data indicate it reacts with the lipoic acid binding region that is contained within the two proteins.

MSP07 Western Blotting Detection of PDH-E1α.
$250.00
100µg of monoclonal antibody reactive against PDH subunit E1α. Human heart mitochondria are also included as a Western blotting control.

Advancing vital discoveries in mitochondrial research.

Western Blotting Products

MitoScience LLC