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Western Blotting
Products
Immunocytochemistry Products
Immunocapture
Products

Mitosciences is proud to provide all of the monoclonal antibody solutions listed below. However for a categorized breakdown of antibodies by functional complex please use the drop down menu under products (above). For a breakdown of antibodies by utility, please click on Western blotting, Immunocyto chemistry or Immunocapture applications listed to the left.

MS101-010 Complex-I Immunocapture Kit
$295.00
100 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25µg of Complex-I (NADH dehdydrogenase) from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

Click to download the specifications PDF

MS101c Complex I Immunocapture mAb
$270.00
100 µg monoclonal antibody which can immunocapture ~25µg of Complex-I (NADH dehdydrogenase) from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MS102-200 Complex-I Subunit Western Blotting Kit
$375.00
Kit MS102 contains a total of 200µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex I subunits NDUFA9, NDUFS3, 20 kDa (likely ND6) along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex I purity or to conveniently determine the levels of 3 key Complex I subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS111, MS110, MS105 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together

MS102-400 Complex-I Subunit Western Blotting Kit
$695.00
Kit MS102 contains a total of 400µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex I subunits NDUFA9, NDUFS3, 20 kDa (likely ND6) along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex I purity or to conveniently determine the levels of 3 key Complex I subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS111, MS110, MS105 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS103 Western Blotting and Immunocytochemistry Detection of GRIM-19
$250.00
100µg of monoclonal antibody reactive against the 19 kDa Complex I (NADH dehydrogenase) subunit GRIM-19. Human heart mitochondria are also included as a Western blotting control. MS103 also labels GRIM-19 in immunocytochemical applications. Interestingly GRIM-19 is also present in the nucleus and cytoplasm. Its expression is necessary for interferonβ/retinoic acid induced tumor mortality.

MS104 Western Blotting Detection of NDUFS4
$250.00
100µg of monoclonal antibody reactive against the 22 kDa Complex I (NADH dehydrogenase) subunit NDUFS4. Human heart mitochondria are also included as a Western blotting control.

MS105 Western blotting Detection of Complex I 20 kDa (likely ND6)
$250.00
100µg of monoclonal antibody reactive against a Complex I (NADH dehydrogenase) 20 kDa subunit. Preliminary data (2D blotting and Rho0 blotting) indicate this antibody is reactive against the mtDNA encoded ND6 subunit. Human heart mitochondria are also included as a Western blotting control.

MS107 Western blotting and immunocytochemistry Detection of Complex I 15 kDa (likely NDUFA6)
$250.00
100µg of monoclonal antibody reactive against a 15 kDa Complex I (NADH dehydrogenase) subunit. Preliminary data(2D blotting) indicate this subunit is the NDUFA6 subunit. Human heart mitochondria are also included as a Western blotting control. MS107 also labels in immunocytochemical applications.

MS108 Western blotting Detection of NDUFB6
$250.00
100µg of monoclonal antibody reactive against the 20 kDa Complex I (NADH dehydrogenase) subunit NDUFB6. Human heart mitochondria are also included as a Western blotting control.

MS109 Western blotting Detection of Complex I 8 kDa
$250.00
100µg of monoclonal antibody reactive against an 8 kDa Complex I (NADH dehydrogenase) subunit. Human heart mitochondria are also included as a Western blotting control.

MS110 Western blotting and Immunocytochemistry Detection of NDUFS3
$250.00
100µg of monoclonal antibody reactive against the 30 kDa Complex I (NADH dehydrogenase) subunit NDUFS3. Human heart mitochondria are also included as a Western blotting control. MS110 also labels NDUFS3 in immunocytochemical applications.

MS111 Western blotting Detection of NDUFA9
$250.00
100µg of monoclonal antibody reactive against the 39 kDa Complex I (NADH dehydrogenase) subunit NDUFA9. Human heart mitochondria are also included as a Western blotting control.

MS112 Western blotting Detection of NDUFS3
$250.00
100µg of monoclonal antibody reactive against the 30 kDa Complex I (NADH dehydrogenase) subunit NDUFS3 in humans and C.elegans. Human heart mitochondria are also included as a Western blotting control.

MS201-010 Complex-II Immunocapture Kit
$295.00
100 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~5µg of Complex-II from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MS201c Complex II Immunocapture mAb
$270.00
100 µg monoclonal antibody which can immunocapture ~5µg of Complex-II from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MS202-300 Complex-II Subunit Western Blotting Kit
$375.00
Kit MS202 contains a total of 300µg of antibodies and is a premixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 3 mAbs (MS204, MS203 and MS502, respectively) can be bought seperately at a 10% discount when all three are ordered together

MS202-600 Complex-II Subunit Western Blotting Kit
$695.00
Kit MS202 contains a total of 600µg of antibodies and is a premixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 3 mAbs (MS204, MS203 and MS502, respectively) can be bought seperately at a 10% discount when all three are ordered together.

MS203 Western blotting and Immunocytochemical detection of Complex II 30 kDa Ip
$250.00
100µg of monoclonal antibody reactive against the Complex II 30 kDa Ip subunit in Western blotting and immunocytochemical applications.

MS204 Western blotting and Immunocytochemical detection of Complex II 70 kDa Fp antibody
$250.00
100µg of monoclonal antibody reactive against the Complex II 70 kDa Fp subunit in Western blotting and immunocytochemical applications.

MS301-010 Complex-III Immunocapture Kit
$295.00
100 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~10µg of Complex-III from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MS301c Complex III Immunocapture mAb
$270.00
100 µg monoclonal antibody which can immunocapture ~10µg of Complex-I from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MS302-200 Complex-III Subunit Western Blotting Kit
$375.00
Kit MS302 contains a total of 200µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex III subunits Core1, 25 kDa Rieske FeS, 10 kDa subunit along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex I purity or to conveniently determine the levels of 3 key Complex III subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS303, MS305, MS306 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS302-400 Complex-III Subunit Western Blotting Kit
$695.00
Kit MS302 contains a total of 400µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex III subunits Core1, 25 kDa Rieske FeS, 10 kDa subunit along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex I purity or to conveniently determine the levels of 3 key Complex III subunits in patient cell lines relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS303, MS305, MS306 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS303 Western blotting detection of Complex III Core 1 protein
$250.00
100µg of monoclonal antibody reactive against the complex III protein core 1. Human heart mitochondria are also included as a Western blotting control.

MS304 Western blotting and Immunocytochemical detection of Complex III Core 2 protein
$250.00
100µg of monoclonal antibody reactive against the Complex III protein core 2. Human heart mitochondria are also included as a Western blotting control. MS304 also labels core 2 in immunocytochemical applications.

MS305 Western blotting detection of Complex III Rieske Protein
$250.00
100µg of monoclonal antibody reactive against the complex III Rieske subunit. Human heart mitochondria are also included as a Western blotting control.

MS306 Western blotting detection of Complex III 10 kDa protien
$250.00
100µg of monoclonal antibody reactive against a complex III 10 kDa subunit. Human heart mitochondria are also included as a Western blotting control.

MS401-010 Complex-IV Immunocapture Kit
$295.00
100 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~10µg of Complex-IV from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MS401c Complex IV Immunocapture mAb
$270.00
100 µg monoclonal antibody which can immunocapture ~10µg of Complex-IV from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MS402-300 Complex-IV Subunit Western Blotting Kit
$375.00
Kit MS402 contains a total of 300µg of antibodies and is a premixed solution of 4 monoclonal antibodies against COXI, COX IV, COX VIc along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex IV purity or to conveniently determine the levels of 3 key Complex IV subunits in patient samples relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS404, MS407, MS414 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS402-600 Complex-IV Subunit Western Blotting Kit
$695.00
Kit MS402 contains a total of 600µg of antibodies and is a premixed solution of 4 monoclonal antibodies against COXI, COX IV, COX VIc along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex IV purity or to conveniently determine the levels of 3 key Complex IV subunits in patient samples relative to the control protein (Complex V α). Alternatively all 4 mAbs (MS404, MS407, MS414 and MS502, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS403 Western Blotting Detection of SURF-1
$250.00
100µg of monoclonal antibody reactive against the complex IV assembly factor SURF-1. Cellular levels of SURF-1 are highest in growing cultured cells.

MS404 Western Blotting and Immunocytochemistry Detection of C-IV subunit I
$250.00
100µg of monoclonal antibody reactive against the COX, Complex IV, subunit I. Human heart mitochondria are also included as a Western blotting control. MS404 also labels COX I in immunocytochemical applications.

MS405 Western Blotting and Immunocytochemistry Detection of C-IV subunit II
$250.00
100µg of monoclonal antibody reactive against the COX, Complex IV, subunit II. Human heart mitochondria are also included as a Western blotting control. MS405 also labels COX II in immunocytochemical applications.

MS406 Western Blotting Detection of C-IV subunit III
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit III. Human heart mitochondria are also included as a Western blotting control.

MS407 Western Blotting and Immunocytochemistry Detection of C-IV subunit IV
$250.00
100µg of monoclonal antibody reactive against the COX, Complex IV, subunit IV. Human heart mitochondria are also included as a Western blotting control. MS407 also labels COX IV in immunocytochemical applications. MS408 also binds COX IV.

MS408 (alternate to 407) Western Blotting and Immunocytochemistry Detection of C-IV subunit IV
$250.00
100µg of monoclonal antibody reactive against the COX, Complex IV, subunit IV. Human heart mitochondria are also included as a Western blotting control. MS408 also labels COX IV in immunocytochemical applications. MS407 also binds COX IV.

MS409 Western Blotting and Immunocytochemistry Detection of C-IV subunit Va
$250.00
100µg of monoclonal antibody reactive against the COX, Complex IV, subunit Va. Human heart mitochondria are also included as a Western blotting control. MS409 also labels COX Va in immunocytochemical applications.

MS410 Western Blotting Detection of C-IV subunit Vb
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit Vb. Human heart mitochondria are also included as a Western blotting control.

MS411 Western Blotting Detection of C-IV subunit VIaH
$250.00
250µg of monoclonal antibody reactive against the COX (complex IV) subunit VIa and specific to the 'heart' isoform. Bovine heart mitochondria are also included as a Western blotting control.

MS412 Western Blotting Detection of C-IV subunit VIaL
$250.00
250µg of monoclonal antibody reactive against the COX (complex IV) subunit VIa and specific to the 'liver' isoform.

MS413 Western Blotting Detection of C-IV subunit VIb
$250.00
100µg of monoclonal antibody reactive against the COX (complex IV) subunit VIb. Human heart mitochondria are also included as a Western blotting control.

MS414 Western Blotting and Immunocytochemistry Detection of C-IV subunit VIc
$250.00
100µg of monoclonal antibody reactive against the COX, Complex IV, subunit VIc. Human heart mitochondria are also included as a Western blotting control. MS414 also labels COX II in immunocytochemical applications.

MS415 Western Blotting Detection of C-IV subunit VIIaH&VIIaL;
$250.00
250µg of monoclonal antibody reactive against the COX (complex IV) subunit VIIa and reacting against both 'liver' and 'heart' isoforms. Bovine heart mitochondria are also included as a Western blotting control.

MS416 Western Blotting Detection of C-IV subunit VIIb
$250.00
250µg of monoclonal antibody reactive against the COX (complex IV) subunit VIIb. Bovine heart mitochondria are also included as a Western blotting control.

MS501-025 Complex-V Immunocapture Kit
$295.00
This kit contains a monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25µg of Complex-V from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MS501c Complex V Immunocapture mAb
$270.00
100 µg monoclonal antibody which can immunocapture up to 25µg of Complex-V from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MS502 Western Blotting and Immunocytochemistry Detection of C-V α.
$250.00
100µg of monoclonal antibody reactive against the F1F0 ATP synthase, Complex V α subunit. Human heart mitochondria are also included as a Western blotting control. MS502 also labels α in immunocytochemical applications.

MS503 Western Blotting and Immunocytochemistry Detection of C-V β
$250.00
100µg of monoclonal antibody reactive against the F1F0 ATP synthase, Complex V β subunit. Human heart mitochondria are also included as a Western blotting control. MS503 also labels β in immunocytochemical applications.

MS504 Western Blotting and Immunocytochemistry Detection of C-V subunit d
$250.00
100µg of monoclonal antibody reactive against the F1F0 ATP synthase, Complex V d subunit. Human heart mitochondria are also included as a Western blotting control. MS504 also labels the d subunit in immunocytochemical applications.

MS505 Western Blotting and Immunocytochemistry Detection of C-V subunit OSCP
$250.00
100µg of monoclonal antibody reactive against the F1F0 ATP synthase, Complex V OSCP subunit. Human heart mitochondria are also included as a Western blotting control. MS505 also labels the OSCP subunit in immunocytochemical applications.

MS506 Western Blotting Detection of C-V IF1 subunit
$250.00
100µg of monoclonal antibody reactive against complex V IF1 subunit. The antibody shows in Western blotting of whole mitochondria shows reactivity with two bands at 10 kDa and at 18 kDa, but in isolated F1F0 only the band at 10 kDa is observed (IF1). The 18 kDa band is the subunit IV of Complex IV. Human heart mitochondria are also included as a Western blotting control.

MS507 Western Blotting and Immunocytochemistry Detection of C-Vα with broad cross-reactivity
$250.00
100µg of monoclonal antibody reactive against the F1F0 ATP synthase, Complex V α subunit. This mAb has broad species cross-reactivity including animal and plant subunits. Human heart mitochondria are also included as a Western blotting control. MS507 also labels the α subunit in immunocytochemical applications.

MS508 Western Blotting and Immunocytochemistry Detection of C-V F6 subunit
$250.00
100µg of monoclonal antibody reactive against the F1F0 ATP synthase, Complex V F6 subunit. Human heart mitochondria are also included as a Western blotting control. MS508 also labels the F6 subunit in immunocytochemical applications.

MS509-200 Complex V Subunit Western Blotting Kit
$375.00
Kit MS509 contains a total of 200µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex V subunits F1α, OSCP,F6 along with an antibody against Complex I NDUFA9 as a control. This cocktail can be used to verify isolated Complex V purity or to conveniently determine the levels of 3 key Complex V subunits in patient cell lines relative to the control protein (Complex I NDUFA9). Alternatively all 4 mAbs (MS502, MS505, MS508 and MS111, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS509-400 Complex V Subunit Western Blotting Kit
$695.00
Kit MS509 contains a total of 400µg of antibodies and is a premixed solution of 4 monoclonal antibodies against Complex V subunits F1α, OSCP,F6 along with an antibody against Complex I NDUFA9 as a control. This cocktail can be used to verify isolated Complex V purity or to conveniently determine the levels of 3 key Complex V subunits in patient cell lines relative to the control protein (Complex I NDUFA9). Alternatively all 4 mAbs (MS502, MS505, MS508 and MS111, respectively) can be bought seperately at a 10% discount when all four are ordered together.

MS601-300 Total OXPHOS Complexes Detection Kit
$375.00
Kit MS601 contains a total of 300µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes. Human heart mitochondria are also included as a Western blotting control.

MS601-600 Total OXPHOS Complexes Detection Kit
$695.00
Kit MS601 contains a total of 600µg of antibodies and is apremixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes. Human heart mitochondria are also included as a Western blotting control.

MS602 OXPHOS/PDH Immunocytochemistry Kit
$495.00
A cocktail of 220µg antibodies comprised of six mAbs packaged individually. Each antibody is against a labile subunit of each of the 5 OXPHOS complexes and PDH. Each mAb is provided in a 50µl aliquot at a 200x concentration, to allow reconstitution of each mAb in 10 ml working volume.

MS801 Human heart mitochondrial sample for Western blotting control
$100.00
100µg of human heart mitochondria solubilized in SDS-PAGE loading buffer and supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.

MS802 Bovine heart mitochondrial sample for immunocapture control
$100.00
2 mg of bovine heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications.

MSA01-010 ANT Immunocapture Kit
$295.00
100 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ANT complex from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MSA01c ANT immunocapture mAb
$270.00
100 µg monoclonal antibody which can immunocapture ANT from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MSA02 Western Blotting and Immunocytochemical Detection of ANT
$250.00
100µg of monoclonal antibody reactive against the adenine nucleotide translocase (ANT). Human heart mitochondria are also included as a Western blotting control. MSA02 also labels ANT in immunocytochemical applications.

MSA03 Western Blotting Detection of Porin
$250.00
100µg of monoclonal antibody reactive against human porin (VDAC1) for Western blotting applications. Human heart mitochondria are also included as a Western blotting control.

MSA04 Western Blotting and Immunocytochemical Detection of Cylophilin D
$250.00
100µg of monoclonal antibody reactive against the human Cyclophilin 'D' (P30405). Human heart mitochondria are also included as a Western blotting control. MSA04 also labels Cyclophilin D in immunocytochemical applications.

MSA05 Immunocytochemical Detection of Porin
$250.00
100µg of monoclonal antibody reactive against human porin (VDAC1)for immunocytochemistry.

MSP01 - 010 PDH Immunocapture Kit
$295.00
100 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~5µg of PDH complex from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MSP01c PDH Immunocapture mAb
$270.00
100 µg monoclonal antibody which can immunocapture PDH from heart mitochondria. Also included are 2mg of bovine heart mitochondria for control immunocapture.

MSP02 - 100 PDH Subunit Western Blotting Kit
$295.00
100µl of an antibody cocktail containing a total of 150µg of monoclonal antibodies reactive against PDH subunits. Human heart mitochondria are also included as a Western blotting control.

MSP02 - 250 PDH Subunit Western Blotting Kit
$645.00
250µl of an antibody cocktail containing a total of 375µg of monoclonal antibodies reactive against PDH subunits. Human heart mitochondria are also included as a Western blotting control.

MSP03 Western blotting and Immunocytochemistry Detection of PDH-E1α.
$250.00
100µg of monoclonal antibody reactive against the PDH E1α subunit. Human heart mitochondria are also included as a Western blotting control. MSP03 also labels E1α in immunocytochemical applications.

MSP04 Western Blotting Detection of PDH - E1β
$250.00
100µg of monoclonal antibody reactive against PDH subunit E1β. Human heart mitochondria are also included as a Western blotting control.

MSP05 Western Blotting and Immunocytochemical detection of PDH-E2
$250.00
100µg of monoclonal antibody reactive against the PDH E2; subunit. Human heart mitochondria are also included as a Western blotting control. MSP05 also labels E2 in immunocytochemical applications.

MSP06 Western Blotting and Immunocytochemical Detection of PDH-E2/E3bp
$250.00
100µg of monoclonal antibody reactive against both the PDH E2 and E3 binding protien subunits. Preliminary data suggest that this antibody recognizes bound lipoic acid. Human heart mitochondria are also included as a Western blotting control. MSP06 also labels E2 & E3bp in immunocytochemical applications.

MSP07 Western blotting and Immunocytochemistry Detection of PDH-E1α.
$250.00
100µg of monoclonal antibody reactive against the PDH-E1α subunit. Human heart mitochondria are also included as a Western blotting control. MSP07 also labels E1α in immunocytochemical applications.

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