All Rapid Microplate Kits



A Novel Approach to Measuring Cytochrome c Oxidase, ATP Synthase, and PDH Activity and Quantity


MitoSciences offers MitoProfile rapid immunocapture microplate assays that provide a fast, efficient and accurate method for measuring the activity and quantity of either cytochrome c oxidase, ATP synthase or PDH.

Given the success of our previous standard microplate assay kits, MitoSciences' rapid microplate assay kits provide improved ease of use for researchers, while still retaining the same high quality of assay.

With the rapid microplate assay kits, the microplate comes pre-coated with antibody, thus reducing the number of required kit components and steps within the protocol, creating a simple, fast assay.

The rapid microplate assays offer several benefits and trade-offs:

• FEATURES: Microplates are fully prepped, with antibody dried in the wells.

• BENEFITS: Fewer required protocol steps, producing a simpler, faster assay. ALL wells are now measurement wells (no nulls), allowing for 96 measurements (as opposed to a typical 75 measurements)

• TRADE-OFFS: Wells are pre-defined, disallowing the researcher to define the wells.



As well, our microplate assays offer many advantages over traditional assay methods or polarography:

• VERSATILITY: PDH can be isolated from whole tissue samples or even whole cultured cells without the need to isolate mitochondria.

• SPEED & EFFICIENCY: 1-96 samples are measured simultaneously.

• ACCURACY & REPRODUCIBILITY: With so many wells available for repeats, accuracy is assured with no extra effort.


Rapid Version Microplate Assay Kits


MS441 Rapid Microplate Assay Kit for Human Complex IV Activity
A rapid activity microplate kit that is used to determine the activity of cytochrome c oxidase in a human sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS442 Rapid Microplate Assay Kit for Human Complex IV Quantity
A rapid activity microplate kit that is used to determine the amount of cytochrome c oxidase in a human sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS443 Rapid Microplate Assay Kit for Human Complex IV Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both the activity and quantity of cytochrome c oxidase in a human sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS444 Rapid Microplate Assay Kit for Mouse Complex IV Activity
A 96-well activity microplate kit that is used to determine the activity of cytochrome c oxidase in a mouse sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS445 Rapid Microplate Assay Kit for Mouse Complex IV Quantity
A 96-well activity microplate kit that is used to determine the amount of cytochrome c oxidase in a mouse sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS446 Rapid Microplate Assay Kit for Mouse Complex IV Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both the activity and quantity of cytochrome c oxidase in a mouse sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS447 Rapid Microplate Assay Kit for Rat Complex IV Activity
A 96-well activity microplate kit that is used to determine the activity of cytochrome c oxidase in a rat sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS541 Rapid Microplate Assay Kit for ATP synthase (Complex V) Activity
A rapid activity microplate kit that is used to determine the activity of ATP synthase in human and rat samples with great speed and simplicity. Includes buffers, detergent, lipid mix, substrates and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS542 Rapid Microplate Assay Kit for ATP synthase (Complex V) Quantity
A rapid activity microplate kit that is used to determine the amount of ATP synthase in human and rat samples with great speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS543 Rapid Microplate Assay Kit for ATP synthase (Complex V) Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both the activity and quantity of ATP synthase in human and rat samples with great speed and simplicity. Includes buffers, detergent, lipid mix, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MS641 MitoProfile Biogenesis Microplate Assay Kit
MS641 is a high-throughput test for determining whether mitochondrial biogenesis has been up-regulated or down-regulated in human samples. The assay works on very small amounts of sample material and is suitable for screening of antiviral and antibiotic drugs.

MS740 Rapid Microplate Assay Kit for Human Mitochondrial Trifunctional Protein (TFP)
This kit is used to determine the assembled enzyme quantity of Mitochondrial Trifunctional Protein (TFP) in a human sample with speed and simplicity.

MSA41 Rapid Microplate Assay Kit for Cytochrome c Quantity
A 96-well activity microplate kit that is used to determine the amount of cytochrome c in a human, bovine, mouse, or rat samples. Includes detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MSA42 Rapid Microplate Assay Kit for Apoptosis-Inducing Factor (AIF)
AIF is a flavoprotein (67 kDa) localized in the inter-membrane space. It is a multi-functional protein with a vital oxidoreductase function (required for cell survival), an anti-oxidant activity, an assembly function in Complex I (NADH-ubiquinone oxidoreductase complex) formation and an apoptogenic function. AIF has a major role in the caspase-independent apoptosis process. It translocates to the nucleus after mitochondrial release, leading to chromatin condensation and DNA fragmentation.

MSF41 Rapid Microplate Assay Kit for Frataxin
Frataxin is a nuclear-encoded mitochondrial protein. In humans the gene is localized on chromosome 9 and is highly conserved during evolution. The gene is expressed in every cell, although in varying levels in different tissues and during development. Friedreich's Ataxia (FRDA) is an inherited recessive neurodegenerative disorder caused by decreased expression levels of frataxin. FRDA is the most common inherited ataxia and it causes progressive damage to the nervous system, resulting in symptoms ranging from muscle weakness and speech problems to heart disease. About one in 50,000 people in the United States have Friedreich's Ataxia.

MSF42 Recombinant Human Frataxin
Frataxin is a nuclear-encoded mitochondrial protein. In humans the gene is localized on chromosome 9 and is highly conserved during evolution. The gene is expressed in every cell, although in varying levels in different tissues and during development. Friedreich's Ataxia (FRDA) is an inherited recessive neurodegenerative disorder caused by decreased expression levels of frataxin. FRDA is the most common inherited ataxia and it causes progressive damage to the nervous system, resulting in symptoms ranging from muscle weakness and speech problems to heart disease. About one in 50,000 people in the United States have Friedreich's Ataxia.

MSP18 Rapid Microplate Assay Kit for PDH Activity
A rapid activity microplate kit that is used to determine the activity of PDH in a human, bovine, mouse, or rat sample with greater speed and simplicity. Includes buffer, detergent, reagent mix, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MSP19 Rapid Microplate Assay Kit for PDH Quantity
A 96-well activity microplate kit that is used to determine the amount of PDH in a human, bovine, mouse, or rat sample. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

MSP20 Rapid Mitcroplate Assay Kit for PDH Activity and Quantity (Combo)
A combination of MSP18 and MSP19 microplate assay kits that are used to determine both the activity and quantity of PDH in a human, bovine, mouse, or rat sample. Includes buffers, detergent, reagent mix, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

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