All Rapid Microplate Kits
A Novel Approach to Measuring Cytochrome c
Oxidase, ATP Synthase, and PDH Activity and Quantity
MitoSciences offers MitoProfile® rapid
immunocapture microplate assays that provide a fast, efficient and
accurate method for measuring the activity and quantity of either
cytochrome c oxidase, ATP synthase or PDH.
Given the success of our previous standard microplate assay kits,
MitoSciences' rapid microplate assay kits provide improved ease of use
for researchers, while still retaining the same high quality of assay.
With the rapid microplate assay kits, the microplate comes pre-coated
with antibody, thus reducing the number of required kit components and
steps within the protocol, creating a simple, fast assay.
The rapid microplate assays offer several
benefits and trade-offs:
• FEATURES: Microplates are fully prepped, with antibody dried in the
wells.
• BENEFITS: Fewer required protocol steps, producing a simpler, faster
assay. ALL wells are now measurement wells (no nulls), allowing for 96
measurements (as opposed to a typical 75 measurements)
• TRADE-OFFS: Wells are pre-defined, disallowing the researcher to
define the wells.
As well, our microplate assays offer many advantages over traditional
assay methods or polarography:
• VERSATILITY: PDH can be isolated from whole tissue samples or even
whole cultured cells without the need to isolate mitochondria.
• SPEED & EFFICIENCY: 1-96 samples are measured simultaneously.
• ACCURACY & REPRODUCIBILITY: With so many wells available for
repeats, accuracy is assured with no extra effort.
Rapid Version Microplate Assay Kits
MS441
Rapid Microplate Assay Kit for
Human Complex IV Activity
A rapid activity microplate kit that is used to determine the activity
of cytochrome c oxidase in a human sample with greater speed and
simplicity. Includes buffers, detergent, substrates, and 96-well
microplate with monoclonal antibody pre-bound to the wells of the
plate, allowing for a stream-lined assay.
MS442
Rapid Microplate Assay Kit for
Human Complex IV Quantity
A rapid activity microplate kit that is used to determine the amount of
cytochrome c oxidase in a human sample with greater speed and
simplicity. Includes buffers, detergent, substrates, and 96-well
microplate with monoclonal antibody pre-bound to the wells of the
plate, allowing for a stream-lined assay.
MS443
Rapid Microplate Assay Kit for
Human Complex IV Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both
the activity and quantity of cytochrome c oxidase in a human sample
with greater speed and simplicity. Includes buffers, detergent,
substrates, and 96-well microplate with monoclonal antibody pre-bound
to the wells of the plate, allowing for a stream-lined assay.
MS444
Rapid Microplate Assay Kit for
Mouse Complex IV Activity
A 96-well activity microplate kit that is used to determine the
activity of cytochrome c oxidase in a mouse sample with greater speed
and simplicity. Includes buffers, detergent, substrates, and 96-well
microplate with monoclonal antibody pre-bound to the wells of the
plate, allowing for a stream-lined assay.
MS445
Rapid Microplate Assay Kit for
Mouse Complex IV Quantity
A 96-well activity microplate kit that is used to determine the amount
of cytochrome c oxidase in a mouse sample with greater speed and
simplicity. Includes buffers, detergent, substrates, and 96-well
microplate with monoclonal antibody pre-bound to the wells of the
plate, allowing for a stream-lined assay.
MS446
Rapid Microplate Assay Kit for
Mouse Complex IV Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both
the activity and quantity of cytochrome c oxidase in a mouse sample
with greater speed and simplicity. Includes buffers, detergent,
substrates, and 96-well microplate with monoclonal antibody pre-bound
to the wells of the plate, allowing for a stream-lined assay.
MS447
Rapid Microplate Assay Kit for Rat
Complex IV Activity
A 96-well activity microplate kit that is used to determine the
activity of cytochrome c oxidase in a rat sample with greater speed and
simplicity. Includes buffers, detergent, substrates, and 96-well
microplate with monoclonal antibody pre-bound to the wells of the
plate, allowing for a stream-lined assay.
MS541
Rapid Microplate Assay Kit for ATP
synthase (Complex V) Activity
A rapid activity microplate kit that is used to determine the activity
of ATP synthase in human and rat samples with great speed and
simplicity. Includes buffers, detergent, lipid mix, substrates and
96-well microplate with monoclonal antibody pre-bound to the wells of
the plate, allowing for a stream-lined assay.
MS542
Rapid Microplate Assay Kit for ATP
synthase (Complex V) Quantity
A rapid activity microplate kit that is used to determine the amount of
ATP synthase in human and rat samples with great speed and simplicity.
Includes buffers, detergent, substrates, and 96-well microplate with
monoclonal antibody pre-bound to the wells of the plate, allowing for a
stream-lined assay.
MS543
Rapid Microplate Assay Kit for ATP
synthase (Complex V) Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both
the activity and quantity of ATP synthase in human and rat samples with
great speed and simplicity. Includes buffers, detergent, lipid mix,
substrates, and 96-well microplate with monoclonal antibody pre-bound
to the wells of the plate, allowing for a stream-lined assay.
MS641
MitoProfile® Biogenesis Microplate Assay Kit
MS641 is a high-throughput test for determining whether mitochondrial
biogenesis has been up-regulated or down-regulated in human samples.
The assay works on very small amounts of sample material and is
suitable for screening of antiviral and antibiotic drugs.
MS740
Rapid Microplate Assay Kit for
Human Mitochondrial Trifunctional Protein (TFP)
This kit is used to determine the assembled enzyme quantity of
Mitochondrial Trifunctional Protein (TFP) in a human sample with speed
and simplicity.
MSA41
Rapid Microplate Assay Kit for
Cytochrome c Quantity
A 96-well activity microplate kit that is used to determine the amount
of cytochrome c in a human, bovine, mouse, or rat
samples. Includes detergent, substrates, and 96-well microplate with
monoclonal antibody pre-bound to the wells of the plate, allowing for a
stream-lined assay.
MSA42
Rapid Microplate Assay Kit for Apoptosis-Inducing Factor (AIF)
AIF is a flavoprotein (67 kDa) localized in the inter-membrane space.
It is a multi-functional protein with a vital oxidoreductase function
(required for cell survival), an anti-oxidant activity, an assembly
function in Complex I (NADH-ubiquinone oxidoreductase complex)
formation and an apoptogenic function. AIF has a major role in the
caspase-independent apoptosis process. It translocates to the nucleus
after mitochondrial release, leading to chromatin condensation and DNA
fragmentation.
MSF41
Rapid Microplate Assay Kit for Frataxin
Frataxin is a nuclear-encoded mitochondrial protein. In humans the gene
is localized on chromosome 9 and is highly conserved during evolution.
The gene is expressed in every cell, although in varying levels in
different tissues and during development. Friedreich's Ataxia (FRDA) is
an inherited recessive neurodegenerative disorder caused by decreased
expression levels of frataxin. FRDA is the most common inherited ataxia
and it causes progressive damage to the nervous system, resulting in
symptoms ranging from muscle weakness and speech problems to heart
disease. About one in 50,000 people in the United States have
Friedreich's Ataxia.
MSF42
Recombinant Human Frataxin
Frataxin is a nuclear-encoded mitochondrial protein. In humans the gene
is localized on chromosome 9 and is highly conserved during evolution.
The gene is expressed in every cell, although in varying levels in
different tissues and during development. Friedreich's Ataxia (FRDA) is
an inherited recessive neurodegenerative disorder caused by decreased
expression levels of frataxin. FRDA is the most common inherited ataxia
and it causes progressive damage to the nervous system, resulting in
symptoms ranging from muscle weakness and speech problems to heart
disease. About one in 50,000 people in the United States have
Friedreich's Ataxia.
MSP18
Rapid Microplate Assay Kit for PDH
Activity
A rapid activity microplate kit that is used to determine the activity
of PDH in a human, bovine, mouse, or rat sample with greater speed and
simplicity. Includes buffer, detergent, reagent mix, and 96-well
microplate with monoclonal antibody pre-bound to the wells of the
plate, allowing for a stream-lined assay.
MSP19
Rapid Microplate Assay Kit for PDH
Quantity
A 96-well activity microplate kit that is used to determine the amount
of PDH in a human, bovine, mouse, or rat sample. Includes buffers,
detergent, substrates, and 96-well microplate with monoclonal antibody
pre-bound to the wells of the plate, allowing for a stream-lined assay.
MSP20
Rapid Mitcroplate Assay Kit for PDH
Activity and Quantity (Combo)
A combination of MSP18 and MSP19 microplate assay kits that are used to
determine both the activity and quantity of PDH in a human, bovine,
mouse, or rat sample. Includes buffers, detergent, reagent mix,
substrates, and 96-well microplate with monoclonal antibody pre-bound
to the wells of the plate, allowing for a stream-lined assay.
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