Pyruvate dehydrogenase (PDH) subunit E1 alpha monoclonal antibody

Catalog No. MSP03

$325.00 - 100 µg

UniProt Number: P08559
Alternate Names: Pyruvate dehydrogenase E1 component subunit alpha (somatic form), PDHE1-A type I, PDHA1, PDHE1A
Structure and Function: The pyruvate dehydrogenase complex contains three different subunits including the pyruvate dehydrogenase E1 subunit. The PDH complex is responsible for catalyzing the formation of acetyl-CoA and CO2 from pyruvate.
Disease Associations: Enzyme deficiency of the PDH E1 subunit of the PDH complex commonly results in lactic acidosis. There are a wide range of symptoms that characterize this disorder including neonatal death, developmental delay, seizures, ataxia, apnea and X-link Leigh Syndrome (LS). This syndrome presents itself with necrotic lesions that are located symmetrically in both sides the subcortical brain.


Product Specifications
Applications: Western blotting, Immunocytochemistry (heat-induced antigen-retrieval improves signal), In-Cell ELISA, Flow Cytometry
Species Reactivity: human, bovine, rat, mouse
Host Species: mouse
Isotype: IgG1, κ
Clone ID: 9H9AF5
Concentration: 1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 1 µg/mL for Western blotting
5 µg/mL for Immunocytochemistry
8 µg/mL for In-Cell ELISA (0.8 µg/well)
1 µg/mL for Flow Cytometry
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA



WB Images

(click to enlarge)

Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), mouse heart (lane 4), and HepG2 (lane 5) detected with (MSP03) anti-subunit E1 alpha antibody.

ICC Images

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Figure 2. Mitochondrial localization of PDH in cultured, normal human embryonic lung fibroblasts (strain MRC5) labeled with anti-PDH-E1 alpha mAb 9H9AF5 (MSP03) as the primary antibody (5 µg/ml) and an AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 µg/ml).

Flow Images

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Figure 3. HL-60 cells were stained with 1 µg/mL PDH subunit E1 alpha antibody (MSP03) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Immunocytochemistry Protocol

   In-Cell ELISA Protocol

   Flow Cytometry Protocol

   PDH Playbook

   MSDS Sodium Azide



Published Studies Using This Product: Frier et al., 2010. Interactions between the consumption of a high fat diet and fasting in the regulation of fatty acid oxidation enzyme gene expression.

Johnson et al., 2009. Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders.

Rardin et al., 2009. Monitoring phosphorylation of the pyruvate dehydrogenase complex.

Zhou et al., 2009. Activation of c-Jun-N-terminal kinase and decline of mitochondrial pyruvate dehydrogenase activity during brain aging.

Missihoun et al., 2009. Myocardial oxidative stress, osteogenic phenotype, and energy metabolism are differentially involved in the initiation and early progression of delta-sarcoglycan-null cardiomyopathy.

Han et al., 2008. A combined therapeutic approach for pyruvate dehydrogenase deficiency using self-complementary adeno-associated virus serotype-specific vectors and dichloroacetate.

Zhou et al., 2008. c-Jun N-terminal kinase regulates mitochondrial bioenergetics by modulating pyruvate dehydrogenase activity in primary cortical neurons.

Arcelay et al., 2008. Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation.

Margineantu et al., 2007. Hsp90 inhibition decreases mitochondrial protein turnover.

Watson et al., 2007. Restoration of CREB function is linked to completion and stabilization of adaptive cardiac hypertrophy in response to exercise.

Kumar et al., 2006. Activity of pyruvate dehydrogenase A (PDHA) in hamster spermatozoa correlates positively with hyperactivation and is associated with sperm capacitation.


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