Figure 1. An example using the MSP30 to measure PDH activity in human fibroblast sample.
Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 160 µg of fibroblast protein extract in a 100 µL reaction volume (50 µL sample in Sample buffer plus 50 µL of Blocking solution). The dipsticks were developed for 1 hour in Activity buffer.
Figure 2. An example using the MSP30 to measure PDH activity in human fibroblast sample.
Based on the standard curve, 160 µg of protein extract were loaded onto a dipstick for each sample. The figure shows two developed dipsticks, a control sample and a sample from a patient with a mutation in the E1-alpha subunit of PDH. The analysis of the signal intensity and interpolation from the standard curve showed that the patient sample had 8% the PDH activity of the control.