Proteins > Pyruvate dehydrogenase (PDH) > Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit

Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit

Catalog No. MSP30

$325.00 - 30 tests
$595.00 - 90 tests


Product Description

This kit contains dipsticks and necessary components to quantify the activity of the PDH enzyme complex (pyruvate dehydrogenase) from human, bovine, mouse, rat, and pig samples.

Learn more about Dipstick Enzyme Activity Assays >



Specifications
 
Data
 
Documentation
 
Citations

Product Specifications
  
Species Reactivity: human, rat, mouse, bovine, porcine
Storage Conditions: Store dipsticks at room temperature out of direct sunlight in their provided containers.

Store buffers #1 and #2 and detergent at 4°C.

Store Reagent Mix #1 and #2 at -80°C.

Store Reagent Mix #3 in the dark at -20°C.
Country of Origin: USA


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Specifications
 
Data
 
Documentation
 
Citations
Figure 1


(click to enlarge)

Figure 1. An example using the MSP30 to measure PDH activity in human fibroblast sample.

Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 160 µg of fibroblast protein extract in a 100 µL reaction volume (50 µL sample in Sample buffer plus 50 µL of Blocking solution). The dipsticks were developed for 1 hour in Activity buffer.
Figure 2


(click to enlarge)

Figure 2. An example using the MSP30 to measure PDH activity in human fibroblast sample.

Based on the standard curve, 160 µg of protein extract were loaded onto a dipstick for each sample. The figure shows two developed dipsticks, a control sample and a sample from a patient with a mutation in the E1-alpha subunit of PDH. The analysis of the signal intensity and interpolation from the standard curve showed that the patient sample had 8% the PDH activity of the control.


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Specifications
 
Data
 
Documentation
 
Citations

Downloadable Documents


   Technical Data Sheet

   Protocol

   Sample Preparation Guide

   PDH Playbook


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Specifications
 
Data
 
Documentation
 
Citations

Published Studies Using This Product: Krus et al., 2010. Pyruvate dehydrogenase kinase 1 controls mitochondrial metabolism and insulin secretion in INS-1 832/13 clonal beta-cells.

Fogal et al., 2010. Mitochondrial p32 Protein is a Critical Regulator of Tumor Metabolism via Maintenance of Oxidative Phosphorylation.

Stenson et al., 2009. Activation of liver X receptor regulates substrate oxidation in white adipocytes.

Sharma et al., 2009. Role of pyruvate dehydrogenase complex in traumatic brain injury and Measurement of pyruvate dehydrogenase enzyme by dipstick test.

Ristorcelli et al., 2008. Human tumor nanoparticles induce apoptosis of pancreatic cancer cells.


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• PDH Kinase 4 (PDK4) (cat.#MSP44)

• PDH Phosphatase 1 (PDP1)
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• PDH Phosphatase 2 (PDP2)
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• Dipstick Reader (cat.#MS1000)




Click here for a video demonstration of a dipstick assay.



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