Poly [ADP-ribose] polymerase 1 (PARP-1)

UniProt Number: P09874
Alternate Names: Cleaved PARP (Asp214), Cleaved PARP, PARP1, PARP, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1
Structure and Function: PARP-1 is nuclear DNA repair enzyme as component of base excision repair complex. It transfers ADP-ribose units from NAD+ to variety of nuclear proteins involved in chromatin architecture and in DNA metabolism including topoisomerases, histones and PARP itself. This modification follows DNA damages and leads to DNA repair. In apoptosis, PARP-1 is cleaved by activated caspase-3 between Asp214 and Gly215, resulting in the formation of an N-terminal 24 kDa fragment containing most of the DNA binding domain and a C-terminal 89 kDa fragment containing the catalytic domain. The proteolysis of PARP-1 through this cleavage renders the enzyme inactive and this further facilitates apoptotic cell death. Thus the presence of 89 kDa PARP-1 fragment is considered to be an important biomarker of apoptosis. PARP-1 is also cleaved by Caspase-7, Granzyme A and Granzyme B.
Disease Associations: n/a

Monoclonal Antibodies
Cat. No. Name Reactivity Apps. Amount
MS777 PARP-1 (cleaved) antibody Human WB, ICC, ICE, FLOW 100 µg
ab170171 Cleaved PARP [4B5BD2] antibody (Alexa Fluor® 488) Human ICC, FLOW 100 µg

In-Cell ELISA Kits
Cat. No. Name Reactivity Amount
MSA43 PARP-1 (cleaved) In-Cell ELISA Kit (IR) Human 2 x 96 Tests
ab139410 PARP-1 (cleaved) In-Cell ELISA Kit (Colorimetric) Human 96 Tests
ab140362 PARP-1 (cleaved) In-Cell ELISA Kit (Fluorescent) Human 96 Tests
ab131383 H2A.X (pSer139) + Cleaved-PARP Human In-Cell ELISA Kit Human 96 tests

Protein Quantitation Assays
Cat. No. Name Reactivity Amount
ab119690 PARP-1 (cleaved) Human ELISA Kit Human 96 Tests

Western Blot Cocktails
Cat. No. Name Reactivity Amount
ab131385 Anti-GAPDH + H2A.X(S139) +cleaved-PARP antibody [3E8, 9F3 and 4B5] Human 100 µg
ab139417 Cell Cycle and Apoptosis WB Cocktail (pCdk/pHH3/Actin/PARP) Human 100 µg

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