These reactive oxygen species can react with nitric oxide to produce reactive nitrogen species including peroxynitrite. While a significant proportion of the reactive oxygen and nitrogen species diffuse into the cytosol, a major portion remains in the mitochondrion. The levels of reactive oxygen and nitrogen species produced is a function of the activity and more specifically the dysfunction of the OXPHOS chain. The less efficient electron transfer is, the more radicals are produced. Free radical damage of cytosolic proteins increases with increased levels of radical production. Moreover, oxidative and nitrative damage of mitochondrial proteins adds to OXPHOS dysfunction further exacerbating free radical production.

Enhanced oxidative stress is now well documented to occur in a number of degenerative diseases including Parkinson's disease, Alzheimer's and diabetes. MitoSciences has a set of mAbs that can immunocapture all 5 OXPHOS complexes for subsequent analysis of accrued oxidative and nitrative damage, either with antibodies to the modified amino acid or by mass spectrometric analysis.
Monoclonal Antibodies
Cat. No. | Name | Reactivity | Apps. | Amount | Price | |
MS786 | Aconitase antibody | human, rat, mouse | ICC, ICE, IP | 100 µg | $325.00 | |
MS721 | Catalase antibody | human, bovine, rat | ICC, IP, ICE | 100 µg | $325.00 | |
MS703 | Nitrotyrosine antibody | human, mouse, rat, bovine | WB, ICC, IP, ICE | 100 µg | $325.00 | |
MS732 | SOD2 antibody | human, bovine, rat, mouse | ICC, IHC, IP, ICE | 100 µg | $325.00 |
Enzyme Activity Assays
Cat. No. | Name | Reactivity | Amount | Price | |
MS745 |
Aconitase Enzyme Activity Microplate Assay Kit |
all species | 96 tests | $425.00 |
Protein Quantity Assays
Cat. No. | Name | Reactivity | Amount | Price | |
MSFX-24 | Catalase FlexPlex™ Module | human | 4 strips (32 tests) | $125.00 | |
MSFX-12 | SOD2 FlexPlex™ Module | human | 4 strips (32 tests) | $125.00 |
All Immunocapture Kits
Cat. No. | Name | Reactivity | Amount | Price | |
MSA01 | ANT Immunocapture Kit | human, bovine | 250 µg | $375.00 | |
500 µg | $675.00 | ||||
750 µg | $875.00 | ||||
MS501 | ATP synthase Immunocapture Kit | human, mouse, rat, bovine | 250 µg | $375.00 | |
500 µg | $675.00 | ||||
750 µg | $875.00 | ||||
MS101 | Complex I Immunocapture Kit | human, mouse, rat, bovine | 250 µg | $375.00 | |
500 µg | $675.00 | ||||
750 µg | $875.00 | ||||
MS201 | Complex II Immunocapture Kit | human, mouse, rat, bovine | 250 µg | $375.00 | |
500 µg | $675.00 | ||||
750 µg | $875.00 | ||||
MS301 | Complex III Immunocapture Kit | human, mouse, rat, bovine | 250 µg | $375.00 | |
500 µg | $675.00 | ||||
750 µg | $875.00 | ||||
MS401 | Complex IV Human Immunocapture Kit | human, bovine | 250 µg | $375.00 | |
500 µg | $675.00 | ||||
750 µg | $875.00 | ||||
MS402 | Complex IV Rodent Immunocapture Kit | mouse, rat | 250 µg | $375.00 | |
500 µg | $675.00 | ||||
750 µg | $875.00 | ||||
MSP01 | PDH Immunocapture Kit | human, bovine | 250 µg | $375.00 | |
500 µg | $675.00 | ||||
750 µg | $875.00 |