Oxidative Stress


Mitochondria are a primary site of production of free radicals. While more than 98% of the molecular oxygen taken up by cells is fully utilized by cytochrome c oxidase to form water, this enzyme can release partly reduced species. Other enzymes of the respiratory chain, and in particular complexes I and III, also produce partly reduced oxygen species including superoxide.

These reactive oxygen species can react with nitric oxide to produce reactive nitrogen species including peroxynitrite. While a significant proportion of the reactive oxygen and nitrogen species diffuse into the cytosol, a major portion remains in the mitochondrion. The levels of reactive oxygen and nitrogen species produced is a function of the activity and more specifically the dysfunction of the OXPHOS chain. The less efficient electron transfer is, the more radicals are produced. Free radical damage of cytosolic proteins increases with increased levels of radical production. Moreover, oxidative and nitrative damage of mitochondrial proteins adds to OXPHOS dysfunction further exacerbating free radical production.

Mitochondrial Antibodies for Oxidative Stress

Enhanced oxidative stress is now well documented to occur in a number of degenerative diseases including Parkinson's disease, Alzheimer's and diabetes. MitoSciences has a set of mAbs that can immunocapture all 5 OXPHOS complexes for subsequent analysis of accrued oxidative and nitrative damage, either with antibodies to the modified amino acid or by mass spectrometric analysis.


Monoclonal Antibodies
Cat. No. Name Reactivity Apps. Amount Price  
MS786 Aconitase antibody human, rat, mouse ICC, ICE, IP 100 µg $325.00
MS721 Catalase antibody human, bovine, rat ICC, IP, ICE 100 µg $325.00
MS703 Nitrotyrosine antibody human, mouse, rat, bovine WB, ICC, IP, ICE 100 µg $325.00
MS732 SOD2 antibody human, bovine, rat, mouse ICC, IHC, IP, ICE 100 µg $325.00


Enzyme Activity Assays
Cat. No. Name Reactivity Amount Price  
MS745 Aconitase Enzyme Activity
Microplate Assay Kit
all species 96 tests $425.00


Protein Quantity Assays
Cat. No. Name Reactivity Amount Price  
MSFX-24 Catalase FlexPlex™ Module human 4 strips (32 tests) $125.00
MSFX-12 SOD2 FlexPlex™ Module human 4 strips (32 tests) $125.00


All Immunocapture Kits
Cat. No. Name Reactivity Amount Price  
MSA01 ANT Immunocapture Kit human, bovine 250 µg $375.00
500 µg $675.00
750 µg $875.00
MS501 ATP synthase Immunocapture Kit human, mouse, rat, bovine 250 µg $375.00
500 µg $675.00
750 µg $875.00
MS101 Complex I Immunocapture Kit human, mouse, rat, bovine 250 µg $375.00
500 µg $675.00
750 µg $875.00
MS201 Complex II Immunocapture Kit human, mouse, rat, bovine 250 µg $375.00
500 µg $675.00
750 µg $875.00
MS301 Complex III Immunocapture Kit human, mouse, rat, bovine 250 µg $375.00
500 µg $675.00
750 µg $875.00
MS401 Complex IV Human Immunocapture Kit human, bovine 250 µg $375.00
500 µg $675.00
750 µg $875.00
MS402 Complex IV Rodent Immunocapture Kit mouse, rat 250 µg $375.00
500 µg $675.00
750 µg $875.00
MSP01 PDH Immunocapture Kit human, bovine 250 µg $375.00
500 µg $675.00
750 µg $875.00



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