Other Protein-Based Technologies and Services for Mitochondrial Toxicity Studies
Immunohistochemical Detection of Mitochondrial Alterations
Many drug interactions are tissue specific and evident early only in limited regions as small
lesions. This is particularly the case when disruption of mitochondrial biogenesis or
accumulation of post-translational modifications cause cell apoptosis or necrosis. In such
cases it is useful to examine mitochondrial dysfunction at a cell by cell level. MitoSciences
provides monoclonal antibody-based kits for immunocytochemistry of cell cultures and
immunohistochemistry of both paraffin and frozen sections. These kits identify altered
biogenesis as well as changes in morphology in response to drug treatments.
Mitochondrial Proteome Screening
The MitoProfile® technology can identify altered levels and post-translation modification of
any of the 100 proteins that constitute the five OXPHOS complexes and the 4 protein
components of PDH. We have monoclonal antibodies to other mitochondrial proteins
including porin, cyclophilin D and frataxin. For an even broader screen of drug effects on
mitochondrial proteins, we provide differential proteome analysis of the entire organelle
protein complement using a combination of 2D gel electrophoresis along with antibody-
based as well as mass spectrometry based identification of proteins altered by any drug treatment.
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