MSP30 MitoProfile® Dipstick Assay Kit
for PDH Activity
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30 Dipstick Kit: $325.00
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60 Dipstick Kit: $545.00
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Please contact us for volume discounts.
Brief Description & Uses
This kit contains dipsticks and necessary components to quantify the
activity of the PDH enzyme complex (pyruvate dehydrogenase) from human,
bovine, mouse, rat, and pig samples.
For a detailed overview of MitoSciences Dipstick Assay Technology please click here.
Specifications
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Product:
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MitoProfile® Dipstick Assay Kit for PDH Activity
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Catalog #:
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MSP30
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Kits Components:
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Dipsticks, buffers, detergent, and reagent mixes.
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mAb Clone ID:
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15D3 (capture antibody on dipsticks)
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Applications:
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Quantitative analysis of PDH activity
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Antigen Species Cross-Reactivity:
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Human, Bovine, Mouse, Rat, Pig
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Storage:
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Store dipsticks at room temperature out of direct sunlight in their
provided containers. Store buffers #1 and #2 and detergent at 4°C.
Store Reagent Mix #1 and #2 at -80°C. Store Reagent Mix #3 in the dark
at -20°C.
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Country of Origin:
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USA
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Product References
There are no references currently posted for this product.
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References for PDH Dipstick Assay
There are no references currently posted for this product.
Images
The above images show PDH activity in a control individual and in a
patient with a mutation in the E1α subunit of PDH; fibroblast protein
extract was taken from both patients as a sample source. The top set of
images show a standard curve for the control sample while in the lower
set of images PDH activity between the control sample and the patient
is compared. From the standard curve it was determined that the patient
has 8% of the PDH activity as compared with the control. Analysis of
the signal was performed using MitoSciences' immunochromatographic
reader (Hamamatsu ICA-1000).
Immunochromatographic Reader
While visual measurements and scanning on a flatbed scanner can both be
used to evaluate our dipsticks, the most effective quantitative results
are derived when using an immunochromatographic reader that has been
specifically designed to read conjugated gold lateral flow devices.
The reader that we recommend is the Hamamatsu ICA-1000,
which is a simple PC-based system for measuring and analyzing our
dipstick products. We have an agreement in place with Hamamatsu that
allows us to offer this reader directly to our customers. To learn more
about this reader, or to order one, please click here to visit the product page.
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