MSP02 MitoProfile® PDH Western Blotting Kit
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150 µg Kit: $395.00
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300 µg Kit: $695.00
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Please contact us for volume discounts.
Brief Description & Uses
Contains a total of 150 µg or 300 µg respectively of monoclonal
antibodies reactive against PDH subunits. Isolated mitochondria are
available from several species for use as control samples. To see the
complete list of control samples available from MitoSciences, click here.
The PDH Western blotting kit contains four different mAbs reacting
specifically with E1α, E1β, E2 and E2/E3bp. A mAb against the OSCP
subunit of Complex V is included as a control. This mAb mixture can be
used to probe the isolated pyruvate dehydrogenase for integrity and
purity as shown in the figure below. Also included in this kit are
SDS-dissociated human heart mitochondria to use as a control for the
identification of PDH subunits on the gel system chosen for analysis.
Click here to review case studies for this product.
Specifications
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Product:
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MitoProfile® PDH Western Blotting Kit
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Kit Components:
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Premixed cocktail containing 5 monoclonal antibodies (see Table below), HHM control.
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Catalog #:
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MSP02
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Lot #:
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Host Species:
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Mouse
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Concentration:
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1.5 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Suggested Working Concentration:
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Approximately 6 µg/ml for Western blotting
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Applications:
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Western blotting detection of enzyme purity of PDH complex.
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Antigen Species Cross-Reactivity:
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human and bovine
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Storage:
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4°C
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Country of Origin:
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USA
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ANTIGEN
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CAT #
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CLONE ID
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AMOUNT
(µg)
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FINAL CONC.
(µg/ml)
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IMMUNOGEN
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PDH E1α
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MSP03
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9H9AF5
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25
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1
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Human recombinant PDH E1α
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PDH E1β
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MSP04
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17A5E2H8
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62.5
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2.5
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Porcine PDH
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PDH E2
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MSP05
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15D3G9C11
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25
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1
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Porcine PDH
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PDH E2/E3bp
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MSP06
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13G2AE2BH5
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25
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1
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Porcine PDH
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C-V-OSCP
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MS505
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4C11C10D12
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12.5
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0.5
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Recombinant human mature OSCP
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Product References
References for PDH Western Blotting Kit
There are no references currently posted for this product.
Images
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