MSP02 MitoProfile PDH Western Blotting Kit

150 g Kit: $395.00
300 g Kit: $695.00
Please contact us for volume discounts.

Brief Description & Uses

Contains a total of 150 g or 300 g respectively of monoclonal antibodies reactive against PDH subunits. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The PDH Western blotting kit contains four different mAbs reacting specifically with E1α, E1β, E2 and E2/E3bp. A mAb against the OSCP subunit of Complex V is included as a control. This mAb mixture can be used to probe the isolated pyruvate dehydrogenase for integrity and purity as shown in the figure below. Also included in this kit are SDS-dissociated human heart mitochondria to use as a control for the identification of PDH subunits on the gel system chosen for analysis.

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Product: MitoProfile PDH Western Blotting Kit

Kit Components:

Premixed cocktail containing 5 monoclonal antibodies (see Table below), HHM control.

Catalog #: MSP02
Lot #:
Host Species: Mouse
Concentration: 1.5 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: Approximately 6 g/ml for Western blotting
Applications: Western blotting detection of enzyme purity of PDH complex.
Antigen Species Cross-Reactivity: human and bovine
Storage: 4C
Country of Origin: USA

PDH E1α MSP03 9H9AF5 25 1 Human recombinant PDH E1α
PDH E1β MSP04 17A5E2H8 62.5 2.5 Porcine PDH
PDH E2 MSP05 15D3G9C11 25 1 Porcine PDH
PDH E2/E3bp MSP06 13G2AE2BH5 25 1 Porcine PDH
C-V-OSCP MS505 4C11C10D12 12.5 0.5 Recombinant human mature OSCP

Product References

Free Radic Biol Med. 2006 Jun 1;40(11):1960-70.
"Postischemic hyperoxia reduces hippocampal pyruvate dehydrogenase activity."

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References for PDH Western Blotting Kit

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PDH Western blotting results

Downloadable Information
MSP02 Specifications Western Blotting Protocol

Additional Information
Product Images
Product References
Protein References

Related Products
Western Blotting Controls

Secondary Antibodies
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