MSP01c PDH Immunocapture
monoclonal antibody
$295.00 (100 µg)
Please contact us for volume discounts.
Brief Description & Uses
100 µg monoclonal antibody which can immunocapture pyruvate dehydrogenase complex (PDH) from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.
The PDH immunocapture kit allows isolation of PDH from small amounts of tissue. This facilitates the subsequent analysis of the assembly state, activity and post-translational modification of the complex. Pyruvate dehydrogenase is a key enzyme in energy metabolism. It converts pyruvate that has been transported into the mitochondrion into the 2-carbon molecule acetyl-CoA for subsequent breakdown in the Krebs cycle (citric acid cycle). PDH activity is under tight control through phosphorylation and dephosphorylation of the E1α subunit, reactions which involve a set of specific pyruvate dehydrogenase kinases (PDK1-4) and phosphatases respectively. The immunocapture antibody isolates a functionally active complex and a protocol has been described for measuring this activity while the enzyme is still bound to the capture beads or fixed in a 96 well plate. Tightly bound kinases and phosphatases are retained on the complex through the immunocapture step. Release of the protein and subsequent gel electrophoresis allows characterization of the phosphorylation state, which can then be analyzed in more detail by mass spectrometry. Uses for this PDH immunocapture kit include but are not limited to examining alterations in assembly of the complex in genetic PDH deficiency altered PDH functioning in late-onset diabetes (NIDMM) and the response of the enzyme complex to diet, exercise and sepsis.
Specifications
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Product:
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PDH Immunocapture
monoclonal antibody
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Catalog #:
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MSP01c
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Lot #:
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Clone ID:
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15D3G9C11
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Host Species and Isotype:
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Mouse IgG1, κ
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Immunogen:
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Porcine heart PDH
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Concentration:
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1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Suggested Working Concentration:
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100 µg mAb can capture approximately 5 µg PDH complex from 1 mg solubilized bovine heart mitochondria.
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Applications:
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Immunoprecipitation
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Antigen Species Cross-Reactivity:
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human and bovine
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Storage:
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4°C
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Country of Origin:
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USA
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Product References
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References for PDH (Pyruvate dehydrogenase complex)
There are no references currently posted for this product.
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