MSA41 MitoProfile® Rapid Microplate Assay for Cytochrome c Quantity
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Brief Description & Uses
The permeabilization of the mitochondrial outer membrane and the consequent release of cytochrome c and other apoptogenic proteins from the mitochondrial intermembrane space into the cytoplasm is considered a hallmark of many apoptotic pathways leading to cell death. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is of prime interest to many researchers.
Because cytochrome c leaves the apoptotic cell following induction of apoptosis it is being increasingly recognized as a potentially useful circulating extracellular diagnostic and prognostic biomarker for disease conditions in which apoptosis is involved. For an overview of cytochrome c's potential utility as a biomarker click here.
MSA41 is used to determine the amount of cytochrome c in a human, mouse, rat or bovine sample. Cytochrome c is immunocaptured within the wells and the amount is determined by adding a cytochrome c specific antibody conjugated with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue at 600 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. The rate of development of blue color can be followed at 600 nm or the reaction can be stopped at a user defined time by the addition of 100 µl of 1.5N HCl to each well (not supplied) and read at 450 nm.
Included for the performance of this quantity assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Specifications
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Product:
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MitoProfile® Rapid Microplate Assay Kit
for Cytochrome c Quantity
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Catalog #:
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MSA41
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Specificity:
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Human, Bovine, Mouse, Rat
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Applications:
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Cytochrome c quantity measurement
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Storage:
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Store detergent at room temperature, do not chill. All other tubes and the covered microplate should be stored at 4°C.
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Country of Origin:
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USA
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Product References
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References for Cytochrome c
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CART
