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Brief Description & Uses Contains 30 or 60 dipsticks and necessary components to quantify the amount of Apoptosis-Inducing Factor (AIF) from human sample material. The kit includes sufficient materials to generate a standard curve and prepare samples for analysis. AIF is a flavoprotein (67 kDa) localized in the inter-membrane space. It is a multi-functional protein with a vital oxidoreductase function (required for cell survival), an anti-oxidant activity, an assembly function in Complex I (NADH-ubiquinone oxidoreductase complex) formation and an apoptogenic function. AIF has a major role in the caspase-independent apoptosis process. It translocates to the nucleus after mitochondrial release, leading to chromatin condensation and DNA fragmentation. The process of apoptosis, the main type of programmed cell death, is an important function in cell termination, cell homeostasis, tissue development and lymphocyte interactions. For a detailed overview of the MitoProfile® Dipstick Technology please click here. Immunochromatographic Reader While visual measurements and scanning on a flatbed scanner can both be used to evaluate our dipsticks, the most effective quantitative results are derived when using an immunochromatographic reader that has been specifically designed to read conjugated gold lateral flow devices. The reader that we recommend is the Hamamatsu ICA-1000, which is a simple PC-based system for measuring and analyzing our dipstick products. We have an agreement in place with Hamamatsu that allows us to offer this reader directly to our customers. To learn more about this reader, or to order one, please click here to visit the product page. Specifications
Product References
There are no references currently posted for this product.
Have you published research using this product? Let us know and earn a $100.00 discount on your next purchase. References for AIF
AMPIS. 2006 Dec;114(12):867-73
"Immunohistochemical and mutational analysis of apoptosis-inducing factor (AIF) in colorectal carcinomas." Prog. Neurobiol. 2007 Feb;81(3):179-96 "Apoptosis-inducing factor: a matter of neuron life and death." Nature. 1999 Feb;397(6718):387-9 "Molecular characterization of mitochondrial apoptosis-inducing factor." Images The above results show the accuracy of the AIF dipstick kit (MSA31) when measuring reduced amounts of AIF when a sample is diluted in 50% increments. HepG2 cell extract was diluted 1:2 and 1:4 and equal volumes of extract were loaded per dipstick. Graphical analysis was performed using values interpolated from a standard curve. All analysis was done with the MS1000 dipstick reader and GraphPad software.
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