MSA09 AIF (Apoptosis-Inducing Factor) monoclonal antibody
$295.00 (100 µg)
Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.
Brief Description & Uses
100 µg of monoclonal antibody reactive against the 67 kDa AIF
(Apoptosis-Inducing Factor), for Western blotting and
immunocytochemistry applications. Isolated mitochondria are available
from several species for use as control samples. To see the complete
list of control samples available from MitoSciences, click here.
AIF is a flavoprotein (67 kDa) localized in the inter-membrane space.
It is a multi-functional protein with a vital oxidoreductase function
(required for cell survival), an anti-oxidant activity, an assembly
function in Complex I (NADH-ubiquinone oxidoreductase complex)
formation and an apoptogenic function. AIF has a major role in the
caspase-independent apoptosis process. It translocates to the nucleus
after mitochondrial release, leading to chromatin condensation and DNA
fragmentation.
The process of apoptosis, the main type of programmed cell death,
is an important function in cell termination, cell homeostasis, tissue
development and lymphocyte interactions.
Specifications
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Product:
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AIF monoclonal antibody
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Catalog #:
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MSA09
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Lot #:
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Clone ID:
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7F7AB10
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Host Species and Isotype:
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Mouse IgG1, κ
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Immunogen:
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Human heart mitochondria
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Concentration:
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1 mg/ml in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Suggested Working Concentration:
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2-5 µg/ml for Western blotting, 5-10 µg/ml for immunocytochemistry
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Applications:
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Western blotting, Immunocytochemistry
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Antigen Species Cross-Reactivity:
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Human
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Storage:
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4°C
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Country of Origin:
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USA
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Product References
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References for AIF
Images
The MSA09 anti-AIF mAb cross-reacts with a single band in human heart
mitochondria (H) and routinely with 2 bands in human liver mitochondria
(L) in a Western blot. This may be the result of enhanced proteolysis
in liver or a reaction with more than one isoform in this tissue.
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CART
