MS604 MitoProfile® Rodent Total OXPHOS
Complexes Detection Kit


300 µg Kit: $575.00
600 µg Kit: $945.00
Please contact us for volume discounts.


Brief Description & Uses

Contains 300 µg or 600 µg of 5 mAbs against CI subunit NDUFB8 (MS105), CII-30kDa (MS203), CIII-Core protein 2 (MS304) CIV subunit I (MS404) and CV α subunit (MS507) respectively as an optimized premixed cocktail. (MS105 was previously listed as likely CI-ND6). The kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from mouse sources.

Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.

The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols). Note Mouse tissue samples can easily be contaminated with antibodies from the animal's blood. To avoid such background bands, use MS604 in conjunction with a secondary against native antibodies e.g. True blot (www.ebiosciences.com). Also, COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel (see image below).


Specifications

Product: MitoProfile® Rodent Total OXPHOS Complexes
Detection Kit
Kit Components: Premixed cocktail containing 5 monoclonal antibodies (see Table below), mouse control.
Catalog #: MS604
Lot #:
Host Species: Mouse
Concentration: 1.5 mg/mL in HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6.0 µg/mL for Western blotting
Applications: Western blotting detection of enzyme purity or for OXPHOS complex deficiency detection.
Antigen Species Cross-Reactivity: Mouse, Rat, also Human, Bovine
Storage: 4°C
Country of Origin: USA


ANTIGEN CAT # CLONE ID AMOUNT
(µg) 		FINAL CONC.
(µg/ML) 		IMMUNOGEN
C-I-NDUFB8 MS105 20E9DH10C12 100 2 Bovine Complex I
C-II-30 (FeS) MS203 21A11AE7 125 2.5 Bovine Heart Complex II
C-III-Core 2 MS304 13G12AF12BB11 25 0.5 Bovine Heart Complex II,III
C-IV-COXI MS404 1D6E1A8 25 0.5 Human Complex IV, subunit I
C-V-α MS507 15H4C4 25 0.5 Bovine Complex V



Product References

J. Molc. and Cell. Cardio. 2007 Nov;16:3982-9.
"Mitochondrial respiration and membrane potential after low-flow ischemia are not affected by ischemic preconditioning "

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References for Oxidative Phosphorylation

There are no references currently posted for this product.




Images


MS604 tested using MS811 Rat liver mitochondria (included in kit).




MS604 tested using MS811 Rat liver mitochondria. The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively.


Downloadable Information
MS604 Specifications Western Blotting Protocol


Additional Information
• Product Images
• Product References
• Protein References


Related Products
• Western Blotting Controls

• Secondary Antibodies

• MS851 Isolation Kit for Rodent Tissue
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