MS602a MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
$750.00
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Brief Description & Uses
A kit of antibodies, one against each of the 5 OXPHOS complexes and PDH. MitoProfile® Kit MS602a contains 220 µg of mAbs, each provided in 50 µl aliquots at a 200x working concentration. The individual mAbs were chosen because they are against a subunit labile when its complex is not assembled and because they work well in immunocytochemistry. The mAbs are against CI-NDUFB4, CII-30 kDa, CIII-Core 2 protein, CIV-subunit I, CV-OSCP and PDH E1α subunit respectively. (CI-NUDFB4 was previously listed as likely CI-NDUFA6).
Kit MS602a contains the same mabs as in MS602 but also provides mAbs against porin (60 µg) and CV α subunit (120 µg) as controls. Thus while the mAbs to Complexes I-IV and against OSCP of Complex V are type IgG1 or IgG2a, the two control mAbs are type IgG2b. Hence it is possible to perform 2-dye immunocytochemistry as described in detail under protocols, and as shown for case studies of patients with mitochondrial disease (click here). Kit MS602a provides appropriate secondary mabs for the 2-dye protocol i.e. anti-mouse IgG1-FITC (80 µg) anti-mouse IgG2a-FITC (40 µg) and anti-mouse IgG2b-TXRD (120 µg).
The OXPHOS immunocytochemistry kit allows rapid screening of cells or tissues for defective assembly of the OXPHOS and PDH complexes. It requires very few cells and only needle biopsy amounts of tissue.
Specifications
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Product:
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MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
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Kit Components:
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50 µl of each of following mAbs: CI-NDUFB4,
CII-30kDa, CIII-Core protein 2, CIV-subunit I, CV-OSCP and PDH E1 α, 60 µg Porin, 120 µg Complex V α, 80 µg IgG1-FITC, 40 µg IgG2a-FITC and 120 µg IgG2b-TXRD
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Catalog #:
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MS602a
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Lot #:
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Host Species:
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Mouse
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Applications:
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Immunocytochemistry (heat-induced antigen-retrieval improves signal in MS602-II, MS602-III and MS602-V).
For double-label experiments, MS507 (C-V-α, an IgG2b) or MSA05 (Porin, an IgG2b) can be used simultaneously with one of the above mAbs.
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Storage:
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4°C
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Country of Origin:
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USA
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Product References
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References for Oxidative Phosphorylation
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Images
Mosaic expression of Complex I and Complex IV in a cell line derived from a patient having a MELAS phenotype. These assembly defects were detected using the MitoProfile® MS602 kit.
Pyruvate Dehydrogenase (PDH) complex assembly defect in a cell line derived from a patient who has a mutation in the PDH E1 alpha subunit. This assembly defect was detected using the MitoProfile® MS602 kit.
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