MS602 MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
$525.00
Please contact us for volume discounts.
Brief Description & Uses
A kit of antibodies, one against each of the 5 OXPHOS complexes and PDH. MitoProfile®
Kit MS602 contains a total of 220 µg of mAbs , each provided in 50 µl
aliquots at a 200x working concentration. The individual mAbs were
chosen because they are against a subunit labile when its complex is
not assembled and because they work well in immunocytochemistry. The
mAbs are against CI-NDUFB4, CII-30kDa, CIII-Core protein 2, CIV-subunit
I, CV-OSCP and PDH E1 α subunit respectively. (CI-NUDFB4 was previously
listed as likely CI-NDUFA6).
Kit MS602a contains the same mabs as in
MS602 but also provides mAbs against porin (60 µg) and CV-alpha subunit
(120 µg) as controls. Thus while the mAbs to Complexes I-IV and against
OSCP of Complex v are type IgG1 or IgG2a, the two control mAbs are type
IgG2b. Hence it is possible to perform 2-dye immunohistochemistry as
described in detail under protocols, and as shown for case studies of
patients with mitochondrial disease (click here). Kit MS602a
provides appropriate secondary mabs for the 2 dye protocol i.e.
anti-mouse IgG1-FITC (80 µg - fluoresces green) anti-mouse IgG 2a-FITC
(40 µg - fluoresces green) and anti-mouse IgG2b-TXRD (120 µg -
fluoresces red).
The OXPHOS immunocytochemistry kit allows rapid screening of cells
or tissues for defective assembly of the OXPHOS and PDH complexes. It
requires very few cells and only needle biopsy amounts of tissue.
Specifications
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Product:
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MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
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Kit Components:
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50 µl of each of the following mAbs: CI-NDUFB4,
CII-30kDa, CIII-Core protein 2, CIV-subunit I, CV-OSCP and PDH E1 α
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Catalog #:
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MS602
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Lot #:
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Host Species:
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Mouse
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Applications:
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Immunocytochemistry (heat-induced antigen-retrieval improves signal in MS602-II, MS602-III and MS602-V).
For
double-label experiments, MS507 (C-V-α, an IgG2b) or MSA05 (Porin, an
IgG2b) can be used simultaneously with one of the above mAbs.
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Storage:
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4°C
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Country of Origin:
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USA
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Product References
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References for OXPHOS/PDH Immunocytochemistry Kit
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Images
Mosaic expression of Complex I and Complex IV in a cell line derived
from a patient having a MELAS phenotype. These assembly defects were
detected using the MitoProfile® MS602 kit.
Pyruvate Dehydrogenase (PDH) complex assembly defect in a cell line
derived from a patient who has a mutation in the PDH E1 alpha subunit.
This assembly defect was detected using the MitoProfile® MS602 kit.
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