MS602 MitoProfile OXPHOS/PDH Immunocytochemistry Kit


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Brief Description & Uses

A kit of antibodies, one against each of the 5 OXPHOS complexes and PDH. MitoProfile Kit MS602 contains a total of 220 g of mAbs , each provided in 50 l aliquots at a 200x working concentration. The individual mAbs were chosen because they are against a subunit labile when its complex is not assembled and because they work well in immunocytochemistry. The mAbs are against CI-NDUFB4, CII-30kDa, CIII-Core protein 2, CIV-subunit I, CV-OSCP and PDH E1 α subunit respectively. (CI-NUDFB4 was previously listed as likely CI-NDUFA6).

Kit MS602a contains the same mabs as in MS602 but also provides mAbs against porin (60 g) and CV-alpha subunit (120 g) as controls. Thus while the mAbs to Complexes I-IV and against OSCP of Complex v are type IgG1 or IgG2a, the two control mAbs are type IgG2b. Hence it is possible to perform 2-dye immunohistochemistry as described in detail under protocols, and as shown for case studies of patients with mitochondrial disease (click here). Kit MS602a provides appropriate secondary mabs for the 2 dye protocol i.e. anti-mouse IgG1-FITC (80 g - fluoresces green) anti-mouse IgG 2a-FITC (40 g - fluoresces green) and anti-mouse IgG2b-TXRD (120 g - fluoresces red).

The OXPHOS immunocytochemistry kit allows rapid screening of cells or tissues for defective assembly of the OXPHOS and PDH complexes. It requires very few cells and only needle biopsy amounts of tissue.


Product: MitoProfile OXPHOS/PDH Immunocytochemistry Kit
Kit Components: 50 l of each of the following mAbs: CI-NDUFB4,
CII-30kDa, CIII-Core protein 2, CIV-subunit I, CV-OSCP and PDH E1 α
Catalog #: MS602
Lot #:
Host Species: Mouse
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Applications: Immunocytochemistry (heat-induced antigen-retrieval improves signal in MS602-II, MS602-III and MS602-V).
For double-label experiments, MS507 (C-V-α, an IgG2b) or MSA05 (Porin, an IgG2b) can be used simultaneously with one of the above mAbs.
Storage: 4C
Country of Origin: USA

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Mitochondrial Antibody Kits for Immunocytochemistry

Mosaic expression of Complex I and Complex IV in a cell line derived from a patient having a MELAS phenotype. These assembly defects were detected using the MitoProfile MS602 kit.

Pyruvate Dehydrogenase (PDH) complex assembly defect in a cell line derived from a patient who has a mutation in the PDH E1 alpha subunit. This assembly defect was detected using the MitoProfile MS602 kit.

Downloadable Information
MS602 Specifications Immunocytochemistry Protocol

Additional Information
Product Images
Product References
Protein References

Related Products
Secondary Antibodies

MS602a MitoProfile OXPHOS/ PDH Kit
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