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Brief Description & Uses Contains 360 µg or 720 µg of 5 mAbs against CI subunit NDUFB8 (MS105), CII-30kDa (MS203), CIII-Core protein 2 (MS304) CIV subunit II (MS405) and CV α subunit (MS507) respectively as an optimized premixed cocktail. (MS105 was previously listed as likely CI-ND6). The kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western Blotting control. Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress. The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols). Examples of the use of kit MS601 in studies of patients with mitochondrial diseases can be seen by clicking here. Specifications
Product Components
Product References
FEBS Letters 2007 July;581(18):3545-9.
"The mitochondrial inner membrane protein Mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11." Exp. Neuro. 2007 Jun 9;204(2):597-609. "Mitochondrial complex I inhibition in cerebral cortex of immature rats following homocysteic acid-induced seizures." Mitochondria. 2006;6:323-30. "Impaired platelet mitochondrial activity in Alzheimer’s disease and mild cognitive impairment" Diabetes 2006 Dec;55:3411-7. "Genetic Ablation of the c-Cbl Ubiquitin Ligase Domain Results in Increased Energy Expenditure and Improved Insulin Action " Am J Physiol Heart Circ Physiol. 2006 Jun 9 "Alterations in oxidative phosophorylation complex proteins in the hearts of transgenic mice that overexpress the p38 MAP kinase activator, MAP kinase kinase 6" J Neurosci. 2006 May 10;26(19):5256-64. "Parkinson's disease brain mitochondrial complex I has oxidatively damaged subunits and is functionally impaired and misassembled." J Neurosci Methods. 2006 Apr 15;152(1-2):136-43. "Isolation of mitochondria with high respiratory control from primary cultures of neurons and astrocytes using nitrogen cavitation." Proteomics 2006;6(24):6437-46. "Proteomic analysis reveals higher demand for antioxidant protection in embryonic stem cell-derived smooth muscle cells." Have you published research using this product? Let us know and earn a $100.00 discount on your next purchase. References for Oxidative Phosphorylation
There are no references currently posted for this product.
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