MS543 MitoProfile® Rapid Microplate Assay Kit for
ATP synthase (Complex V) Activity and Quantity (Multiplexing)


$395.00

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Brief Description & Uses

This rapid multiplexing microplate kit is used to determine both the activity and quantity of ATP synthase (Complex V) in a human or rat sample. The ratio of the two measurements represents the enzyme's specific activity. The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. Specifically, the conversion of ATP to ADP by ATP synthase is coupled to the oxidative reaction of NADH to NAD+. The formation of NAD+ results in a decrease in absorbance at 340 nm. Subsequently, in these same wells, the quantity of ATP synthase is determined by adding an ATP synthase specific antibody conjugated with alkaline phosphatase. This phosphatase changes the substrate pNPP from colorless to yellow (OD 405 nm) in a time dependent manner proportional to the amount of protein captured in the wells. Included for optimal performance of the assay are buffer, detergent, antibody, substrate, lipid mix and a 96-well microplate pre-coated with monoclonal antibody.


Specifications

Product: MitoProfile® Rapid Microplate Assay Kit for Human ATP synthase (Complex V) Activity and Quantity (Mulitplexing)
Catalog #: MS543
Clone ID (mAbs): 12F4AD8
3D5AB1
Specificity: Human, Rat
Applications: Analysis of ATP synthase activity and quantity
Storage: Buffers, detergents, lipid mixes, and microplates should be stored at 4°C.
Reagent mix and Tube 3 should be stored at -20°C.
Country of Origin: USA



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References for ATP synthase

Neurosci Lett. 2007 Mar;415(3):205-9
"Abnormal levels of prohibitin and ATP synthase in the substantia nigra and frontal cortex in Parkinson's disease."

Biochim Biophys Acta. 2006 Sept-Oct;1757(9-10):1400-5
"Mitochondrial diseases and genetic defects of ATP synthase."

Ann Neurol. 2006 Feb;59(2):265-75
"Subcomplexes of human ATP synthase mark mitochondrial biosynthesis disorders."

Nat Genet. 2002 Apr;30(4):394-9
"Rescue of a deficiency in ATP synthesis by transfer of MTATP6, a mitochondrial DNA-encoded gene, to the nucleus."

Semin Cell Dev Biol. 2001 Dec;12(6):441-8
"Pathogenesis of primary defects in mitochondrial ATP synthesis."


Downloadable Information
MS543 Specifications


Additional Information
• Product References
• Protein References


Related Products
• MS541 MitoProfile® Rapid Microplate Assay Kit for ATP synthase (Complex V) Activity

• MS542 MitoProfile® Rapid Microplate Assay Kit for ATP synthase (Complex V) Quantity
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