MS542 MitoProfile® Rapid Microplate Assay Kit for
ATP synthase (Complex V) Quantity


$325.00

Please contact us for volume discounts.


Brief Description & Uses

This rapid quantity microplate kit is used to determine the amount of ATP synthase (Complex V) in a human or rat sample. The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme amount is determined by adding an ATP synthase specific antibody conjugated with alkaline phosphatase. This phosphatase changes the substrate pNPP from colorless to yellow (OD 405 nm) in a time dependent manner proportional to the amount of protein captured in the wells. Included for optimal performance of the assay are buffer, detergent, antibody, substrate, lipid mix and a 96-well microplate pre-coated with monoclonal antibody.

The rapid quantity microplate can be performed in conjunction with the rapid activity microplate to establish specific activity; this rapid multiplexing microplate (MS543) is available as a separate kit.


Specifications

Product: MitoProfile® Rapid Microplate Assay Kit
for ATP synthase (Complex V) Quantity
Catalog #: MS542
Clone ID (mAbs): 12FAD8
3D5AB1
Specificity: Human, Rat
Applications: Analysis of ATP synthase quantity
Storage: Buffers, detergent, lipid mix, and microplate should be stored at 4°C.
Tube 3 should be stored at -20°C.
Country of Origin: USA



Product References

There are no references currently posted for this product.

Have you published research using this product?
Let us know and earn a $100.00 discount on your next purchase.



References for ATP synthase

Neurosci Lett. 2007 Mar;415(3):205-9
"Abnormal levels of prohibitin and ATP synthase in the substantia nigra and frontal cortex in Parkinson's disease."

Biochim Biophys Acta. 2006 Sept-Oct;1757(9-10):1400-5
"Mitochondrial diseases and genetic defects of ATP synthase."

Ann Neurol. 2006 Feb;59(2):265-75
"Subcomplexes of human ATP synthase mark mitochondrial biosynthesis disorders."

Nat Genet. 2002 Apr;30(4):394-9
"Rescue of a deficiency in ATP synthesis by transfer of MTATP6, a mitochondrial DNA-encoded gene, to the nucleus."

Semin Cell Dev Biol. 2001 Dec;12(6):441-8
"Pathogenesis of primary defects in mitochondrial ATP synthesis."


Downloadable Information
MS542 Specifications


Additional Information
• Product References
• Protein References


Related Products
• MS541 MitoProfile® Rapid Microplate Assay Kit for ATP Synthase (Complex V) Activity

• MS543 MitoProfile® Rapid Microplate Assay Kit for ATP Synthase (Complex V) Multiplexing
Home - About Us - Products - Support - Resources - Careers - Contact Us - Shopping Cart

Sales & Customer Support:
1-800-910-6486
[email protected]

Copyright © 2004-2008 MitoSciences Inc. All rights reserved.