MS503 ATP synthase (Complex V) subunit β
monoclonal antibody


$295.00 (100 µg)

Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.


Brief Description & Uses

100 µg of monoclonal antibody reactive against the 52 kDa F1F0 ATP synthase (Complex V) β subunit. MS503 also labels β in immunocytochemical applications. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

An important recent finding is that F1F0 is an ectopic protein in many cells and tissues in addition to its location within mitochondria. In endothelial cells F1F0 acts as an angiostatin receptor. Both synthesis and hydrolysis activity of the ectopic F1F0 are inhibited by angiostatin. The β mAb above (MS503) has been shown to act as an angiostatin mimetic and to inhibit the proliferation of endothelial cells. The anti β subunit mAb (MS503) is able to detect plasma membrane F1F0. More recently it has been reported that in liver cells this protein complex is the HDL receptor. Importantly not all cells have F1F0 on their surface; there is no reactivity of this antibody with the surface of MRC-5 fibroblast cells.


Specifications

Product: ATP synthase (Complex V) subunit β
monoclonal antibody
Catalog #: MS503
Lot #:
Clone ID: 3D5AB1
Host Species and Isotype: Mouse IgG1, κ
Immunogen: Human Heart Mitochondria
Concentration: 1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 0.5 µg/ml for Western blotting, 1-2 µg/ml for Immunocytochemistry
Applications: Western blotting, Immunocytochemistry
Antigen Species Cross-Reactivity: human, bovine, mouse, rat and C. elegans
Storage: 4°C
Country of Origin: USA



Product References

Genome Res. 2007 Aug;17:1319-1326.
"Direct linkage of mitochondrial genome variation to risk factors for type 2 diabetes in conplastic strains."

Biochem. J. 2007 Mar;404:373-81.
"Bystin in human cancer cells: intracellular localization and function in ribosome biogenesis."

J. Bio. Chem. 2007 Feb;282(8):5910-18.
"Knockdown of Mitochondrial Heat Shock Protein 70 Promotes Progeria-like Phenotypes in Caenorhabditis elegans."

J. Bio. Chem. 2007 Feb;282(8):5670-81.
"Identification of a Novel Mitochondrial Complex Containing Mitofusin 2 and Stomatin-like Protein 2."

DISS. ETH NO. 16679. 2006.
"Apolipopotein A-I Transcytosis Through Aortic Endothelial Cells."

Have you published research using this product?
Let us know and earn a $100.00 discount on your next purchase.



References for ATP synthase (Complex V) subunit β

MitoNews, Volume 01, Issue 11
"Novel roles of the ATP Synthase on the surface of cells"

MitoNews, Volume 01, Issue 06
"The ATP Synthase: An Enzyme That Does More That You Are Probably Aware"



Images




Complex V subunit ? Immunocytochemistry Image


Downloadable Information
MS503 Specifications Western Blotting Protocol Immunocytochemistry Protocol


Additional Information
• Product Images
• Product References
• Protein References


Related Products
• Western Blotting Controls

• Secondary Antibodies

• MS851 Isolation Kit for Rodent Tissue
Home - About Us - Products - Support - Resources - Careers - Contact Us - Shopping Cart

Sales & Customer Support:
1-800-910-6486
[email protected]

Copyright © 2004-2008 MitoSciences Inc. All rights reserved.