MS502 ATP synthase (Complex V) subunit α
monoclonal antibody


$295.00 (100 µg)

Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.


Brief Description & Uses

100 µg of monoclonal antibody reactive against the 55 kDa F1F0 ATP synthase (Complex V) α subunit. MS502 also labels α in immunocytochemical applications. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here. An important recent finding is that F1F0 is an ectopic protein in many cells and tissues in addition to its location within mitochondria. In endothelial cells F1F0 acts as the angiostatin receptor. Both synthesis and hydrolysis activity of the ectopic F1F0 are inhibited by angiostatin. The α mAb (MS502) has been shown to act as an angiostatin mimetic and to inhibit the proliferation of endothelial cells. More recently it has been reported that in liver cells this protein complex is the HDL receptor. Importantly not all cells have F1F0 on their surface; there is no reactivity of this antibody with the surface of MRC-5 fibroblast cells.


Specifications

Product: ATP synthase (Complex V) subunit α
monoclonal antibody
Catalog #: MS502
Lot #:
Clone ID: 7H10BD4F9
Host Species and Isotype: Mouse IgG2b, κ
Immunogen: Bovine Complex V
Concentration: 1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 1-2 µg/ml for Western blotting, 5 µg/ml for immunocytochemistry
Applications: Western blotting, Immunocytochemistry
Antigen Species Cross-Reactivity: human, bovine, mouse, rat, and Zebrafish
Storage: 4°C
Country of Origin: USA



Product References

PloS One 2007 Oct;2(10):e1066.
"Hsp90 Inhibition Decreases Mitochondrial Protein Turnover."

PNAS. 2007 July;104(28):11655-9.
"U bodies are cytoplasmic structures that contain uridine-rich small nuclear ribonucleoproteins and associate with P bodies ."

Mol Cell Biol. 2006 Feb;26(3):1077-86.
"Nuclear suppression of mitochondrial defects in cells without the ND6 subunit."

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References for ATP synthase (Complex V) subunit α

MitoNews, Volume 01, Issue 11
"Novel roles of the ATP Synthase on the surface of cells"

MitoNews, Volume 01, Issue 06
"The ATP Synthase: An Enzyme That Does More That You Are Probably Aware"



Images

Complex V subunit ? Western Blotting Image




Downloadable Information
MS502 Specifications Western Blotting Protocol Immunocytochemistry Protocol


Additional Information
• Product Images
• Product References
• Protein References


Related Products
• Western Blotting Controls

• Secondary Antibodies

• MS851 Isolation Kit for Rodent Tissue

• MS620 Mitochondria Membrane Integrity Kit
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