MS501c ATP synthase (Complex V)
Immunocapture monoclonal antibody
$295.00 (100 µg)
Please contact us for volume discounts.
Brief Description & Uses
100 µg monoclonal antibody which can immunocapture up to 50 µg of ATP
synthase (Complex V) from heart mitochondria. Isolated mitochondria are
available from several species for use as control samples. To see the
complete list of control samples available from MitoSciences, click here.
The ATP synthase (Complex V) immunocapture kit allows isolation of the
ATP synthase complex (E.C. 3.6.3.14) from small amounts of tissue. This
facilitates subsequent analysis of assembly state and activity. Thus
the enzyme retains olygomycin sensitive ATP hydrolysis activities after
isolation. Finally, the extent of post translational modifications
including oxidative damage can be readily analyzed by proteomic
approaches or antibody detection of these modifications. Potential uses
for the ATP synthase (Complex V) immunocapture kit include but are not
limited to examining alterations of Complex V subunits in inherited
mitochondrial diseases, Alzheimer's disease, angiogenesis, hypertension
and aging.
Specifications
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Product:
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ATP synthase (Complex V) Immunocapture
monoclonal antibody
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Catalog #:
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MS501c
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Lot #:
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Clone ID:
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12F4AD8AF8
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Host Species and Isotype:
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Mouse IgG2b, κ
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Immunogen:
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Bovine Complex V
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Concentration:
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1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Suggested Working Concentration:
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100 µg mAb can capture up to 50 µg ATP synthase (Complex V) from 1 mg solubilized bovine heart mitochondria.
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Applications:
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Immunoprecipitation
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Antigen Species Cross-Reactivity:
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human, bovine, rat and mouse
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Storage:
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4°C
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Country of Origin:
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USA
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Product References
References for ATP synthase (Complex V)
Images
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