MS501 MitoProfile® ATP synthase (Complex V)
Immunocapture Kit


250 µg Kit: $375.00
500 µg Kit: $675.00
750 µg Kit: $875.00
Please contact us for volume discounts.


Brief Description & Uses

Contains a total of 250 µg, 500 µg or 750 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 µg, ~250 µg or ~500 µg respectively of ATP synthase (Complex V) from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The MitoProfile® ATP synthase (Complex V) Immunocapture Kit allows isolation of the ATP synthase complex (E.C. 3.6.3.14) from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for the ATP synthase (Complex V) immunocapture kit include but are not limited to examining alterations of Complex V subunits in inherited mitochondrial diseases, Alzheimer’s disease, angiogenesis, hypertension and aging.


Specifications

Product: MitoProfile® ATP synthase (Complex V) Immunocapture Kit

Kit Components:

250 µg of antibody crosslinked to protein G-agarose beads, BHM control.

Catalog #: MS501
Lot #:
Clone ID: 12F4AD8AF8
Host Species and Isotype: Mouse IgG2b, κ
Immunogen: Bovine Complex V
Concentration: 250 µg of capture antibody crosslinked to 62.5 µl matrix in 400 µl HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 10 µl matrix capture approximately 20 µg ATP synthase (Complex V) from 1 mg solubilized bovine heart mitochondria.
Applications: Immunoprecipitation
Antigen Species Cross-Reactivity: human, bovine, rat and mouse
Storage: 4°C
Country of Origin: USA



Product References

J. Bio Chem. 2007 Jan;282(2):1051-58.
"ATP6 Homoplasmic Mutations Inhibit and Destabilize the Human F1F0-ATP Synthase without Preventing Enzyme Assembly and Oligomerization."

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References for ATP synthase (Complex V)

MitoNews, Volume 01, Issue 11
"Novel roles of the ATP Synthase on the surface of cells"

MitoNews, Volume 01, Issue 06
"The ATP Synthase: An Enzyme That Does More That You Are Probably Aware"



Images



Downloadable Information
MS501 Specifications Immunocapture Protocol


Additional Information
• Product Images
• Product References
• Protein References


Related Products
•Immunocapture Controls

• Secondary Antibodies

• Detergents

• MS851 Isolation Kit for Rodent Tissue

• MS501c ATP synthase (Complex V) Immunocapture mAb
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