MS446 MitoProfile® Rapid Microplate Assay Kit for Mouse
Complex IV Activity and Quantity (Multiplexing)
$395.00
Please contact us for volume discounts.
Brief Description & Uses
This rapid multiplexing microplate kit is used to determine both the activity and quantity of cytochrome c oxidase in a mouse sample with greater speed and simplicity. The ratio of the two is a measure of specific activity. The COX enzyme is immunocaptured within the wells of the microplate and the activity is determined by following the oxidation of reduced Cytochrome c colorimetrically by the absorbance change at 550 nm. Subsequently, in these same wells the quantity of the enzyme can then be determined by adding a COX specific detector antibody and the appropriate alkaline phophatase conjugated antibody; this phosphatase changes a substrate from colorless to yellow at 405 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. Included in this kit for performance of the multiplexing assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Specifications
|
Product:
|
MitoProfile® Rapid Microplate Assay Kit for Mouse
Complex IV Activity and Quantity (Mulitplexing)
|
|
Catalog #:
|
MS446
|
|
Purity:
|
Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
|
|
Specificity:
|
mouse
|
|
Applications:
|
COX activity and quantity measurement.
|
|
Storage:
|
Buffers, detergent substrates, and microplate should be stored at 4°C.
Reagent C (reduced Cytochrome c) and Tube 4 should be stored at -20°C.
|
|
Country of Origin:
|
USA
|
Product References
There are no references currently posted for this product.
Have you published research using this product?
Let us know and earn a $100.00 discount on your next purchase.
References for Cytochrome c oxidase
|
CART
