MS446 MitoProfile® Rapid Microplate Assay Kit for Mouse
Complex IV Activity and Quantity (Multiplexing)


$395.00

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Brief Description & Uses

This rapid multiplexing microplate kit is used to determine both the activity and quantity of cytochrome c oxidase in a mouse sample with greater speed and simplicity. The ratio of the two is a measure of specific activity. The COX enzyme is immunocaptured within the wells of the microplate and the activity is determined by following the oxidation of reduced Cytochrome c colorimetrically by the absorbance change at 550 nm. Subsequently, in these same wells the quantity of the enzyme can then be determined by adding a COX specific detector antibody and the appropriate alkaline phophatase conjugated antibody; this phosphatase changes a substrate from colorless to yellow at 405 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. Included in this kit for performance of the multiplexing assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.


Specifications

Product: MitoProfile® Rapid Microplate Assay Kit for Mouse Complex IV Activity and Quantity (Mulitplexing)
Catalog #: MS446
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Specificity: mouse
Applications: COX activity and quantity measurement.
Storage: Buffers, detergent substrates, and microplate should be stored at 4°C.
Reagent C (reduced Cytochrome c) and Tube 4 should be stored at -20°C.
Country of Origin: USA



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References for Cytochrome c oxidase



Downloadable Information
MS446 Specifications Research Playbook


Additional Information
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