MS442 MitoProfile® Rapid Microplate Assay Kit for
Human Complex IV Quantity


$325.00

Please contact us for volume discounts.


Brief Description & Uses

This rapid quantity microplate kit is used to determine the amount of cytochrome c oxidase in a human sample with greater speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate, and the amount is determined by adding a COX specific antibody conjugated with alkaline phophatase; this phosphatase changes the substrate from colorless to yellow at 405 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. Included in this kit for performance of the quantity assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

The rapid quantity microplate can be performed in conjunction with the rapid activity microplate to establish specific activity. This rapid multiplexing microplate (MS443) is available as a separate kit.


Specifications

Product: MitoProfile® Rapid Microplate Assay Kit
for Human Complex IV Quantity
Catalog #: MS442
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Specificity: Human (and bovine)
Applications: COX quantity measurement
Storage: Buffers, detergent, substrates, and microplate should be stored at 4°C.
Tube 4 should be stored at -20°C.
Country of Origin: USA



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References for Cytochrome c oxidase



Downloadable Information
MS442 Specifications Research Playbook


Additional Information
Product Images
Product References
Protein References


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MS441 MitoProfile® Rapid Microplate Assay Kit for Human Complex IV Activity

MS443 MitoProfile® Rapid Microplate Assay Kit for Human Complex IV Multiplexing
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