MS442 MitoProfile® Rapid Microplate Assay Kit for
Human Complex IV Quantity
$325.00
Please contact us for volume discounts.
Brief Description & Uses
This rapid quantity microplate kit is used to determine the amount of cytochrome c oxidase in a human sample with greater speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate, and the amount is determined by adding a COX specific antibody conjugated with alkaline phophatase; this phosphatase changes the substrate from colorless to yellow at 405 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. Included in this kit for performance of the quantity assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
The rapid quantity microplate can be performed in conjunction with the rapid activity microplate to establish specific activity. This rapid multiplexing microplate (MS443) is available as a separate kit.
Specifications
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Product:
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MitoProfile® Rapid Microplate Assay Kit
for Human Complex IV Quantity
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Catalog #:
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MS442
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Specificity:
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Human (and bovine)
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Applications:
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COX quantity measurement
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Storage:
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Buffers, detergent, substrates, and microplate should be stored at 4°C.
Tube 4 should be stored at -20°C.
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Country of Origin:
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USA
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Product References
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References for Cytochrome c oxidase
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CART
