MS441 MitoProfile® Rapid Microplate Assay Kit for
Human Complex IV Activity


$325.00

Please contact us for volume discounts.


Brief Description & Uses

This rapid activity microplate kit is used to determine the activity of cytochrome c oxidase in a human sample with greater speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm. Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

The rapid activity microplate can be performed in conjunction with the rapid quantity microplate to establish specific activity. This rapid multiplexing microplate (MS443) is available as a separate kit.


Specifications

Product: MitoProfile® Rapid Microplate Assay Kit
for Human Complex IV Activity
Catalog #: MS441
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Specificity: Human (and bovine)
Applications: COX activity measurement
Storage: Buffer, detergent, and microplate should be stored at 4°C.
Reagent C (reduced cytochrome c) should be stored at -20°C.
Country of Origin: USA



Product References

Amer. J. Physiol. - Cell Physiol. 2007 Mar;293:C566-75.
"Estradiol and dihydrotestosterone regulate endothelial cell barrier function after hypergravity-induced alterations in MAPK activity ."

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References for cytochrome c oxidase

MitoNews, Volume 02, Issue 01
"CYTOCHROME c OXIDASE: BOTH CAUSATIVE AND A BIOMARKER OF DISEASE"

MitoNews, Volume 01, Issue 04
"Stress the organ and cytochrome c oxidase levels go up?"



Downloadable Information
MS441 Specifications Research Playbook


Additional Information
• Product Images
• Product References
• Protein References


Related Products
• MS444 MitoProfile® Rapid Microplate Assay Kit for Mouse Complex IV Activity

• MS447 MitoProfile® Rapid Microplate Assay Kit for Rat Complex IV Activity
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