MS430 MitoProfile® Dipstick Assay Kit
for Human Complex IV Activity
30 Dipstick Kit: $325.00
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60 Dipstick Kit: $545.00
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Please contact us for volume discounts.
Brief Description & Uses
Contains 30 or 60 dipsticks and necessary components to quantify the activity of the cytochrome c oxidase enzyme complex from human and bovine samples.
The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.
For a detailed overview of MitoSciences Dipstick Assay Technology please click here.
Immunochromatographic Reader
While visual measurements and scanning on a flatbed scanner can both be
used to evaluate our dipsticks, the most effective quantitative results
are derived when using an immunochromatographic reader that has been
specifically designed to read conjugated gold lateral flow devices.
The reader that we recommend is the Hamamatsu ICA-1000,
which is a simple PC-based system for measuring and analyzing our
dipstick products. We have an agreement in place with Hamamatsu that
allows us to offer this reader directly to our customers. To learn more
about this reader, or to order one, please click here to visit the product page.
Specifications
Product:
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MitoProfile® Dipstick Assay Kit
for Human Complex IV Activity
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Catalog #:
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MS430
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Kit Components:
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Dipsticks, buffers, and developing trays.
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mAb Clone ID:
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31E91B8 (capture antibody on dipsticks)
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Applications:
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Quantitative analysis of cytochrome c oxidase activity
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Antigen Species Cross-Reactivity:
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Human, Bovine
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Storage:
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Store dipsticks and gold-conjugated secondary antibody at room
temperature out of direct sunlight in their provided containers. Store
buffers A, B, and C at 4°C. Store Tube 1 and Tube 2 at -20°C. Store
Tube 3 at room temp.
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Country of Origin:
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USA
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Product References
There are no references currently posted for this product.
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References for Complex IV Dipstick Assays
There are no references currently posted for this product.
Images
The above results show the clear visual difference between
individuals with normal COX enzyme activity compared to a patient with
defects in COX assembly (SURF1 patient). The dipstick results are both
quantitative and highly reproducible (intra-assay error less than 10%).
Data can be analyzed with a simple flat-be scanner and common imaging
software or, for sensitive and reproducible results, a dipstick reader
can be utilized (see our suggested reader: Hamamatsu ICA-1000).
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