MS423 Microplate Assay Kit for Human
Complex IV Activity and Quantity (Multiplexing)


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Brief Description & Uses

The 96-well multiplexing microplate kit is used to determine both the activity and quantity of Cytochrome c oxidase in a human sample. The ratio of the two is a measure of specific activity. The COX enzyme is immunocaptured within the wells of the microplate and the activity is determined by following the oxidation of reduced Cytochrome c colorimetrically by the absorbance change at 550 nm. The amount can then be determined in these same wells by adding a COX specific detector antibody and the appropriate alkaline phophatase conjugated antibody. Also included are reagents and buffers to perform the assay.


Product: Microplate Assay Kit for Human
Complex IV Activity and Quantity (Mulitplexing)
Catalog #: MS423
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Specificity: Human (and bovine)
Applications: COX activity and quantity measurement.
Storage: Antibodies and buffers should be stored at 4°C.
Reagent C (reduced Cytochrome c) and Tube 5 should be stored at -20°C.
Country of Origin: USA

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References for Cytochrome c oxidase

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MS423 Specifications

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Reference Proteins
Secondary Antibodies
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