MS408 Complex IV subunit IV
monoclonal antibody (alternate to 407)
$295.00 (100 µg)
Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.
Brief Description & Uses
100 µg of monoclonal antibody reactive against the 18 kDa COX (Complex
IV) subunit IV. MS408 also labels COX IV in immunocytochemical
applications. Reacts only with human and bovine, but better than MS407
for immunocytochemistry in these species. Isolated mitochondria are
available from several species for use as control samples. To see the
complete list of control samples available from MitoSciences, click here.
Variations in the synthesis of cytochrome c oxidase subunits from the
two genomes (mitochondrial and nuclear) have been described as markers
for several cancers including those of the prostate, and thyroid.
Additionally this ratio has been reported to identify areas of the
brain damaged in Alzheimer’s disease and also to relate to the overall
regulation of mitochondrial biogenesis in exercise and diet. To examine
this ratio in immunocytochemistry we offer two mAbs against
mitochondrially encoded subunits I and II (MS404 and MS405,
respectively) and mAbs against two nuclear encoded subunits IV and Va
(MS408 and MS409, respectively). The latter two are retained in
mitochondria exhibiting heterolpasmy or mosaiscism of altered mtDNA
transcription e.g. in anti-retroviral therapy. Therefore an
immunocytochemical study using a combination of subunits I or II
alongside IV or Va could reveal changes in the levels of mtDNA or mtDNA
encoded proteins.
Specifications
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Product:
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Complex IV subunit IV
monoclonal antibody (alternative to MS407)
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Catalog #:
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MS408
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Lot #:
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Clone ID:
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10G8D12C12
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Host Species and Isotype:
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Mouse IgG2a, κ
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Immunogen:
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Bovine Complex IV subunit IV
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Concentration:
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1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Suggested Working Concentration:
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1 µg/ml for Western blotting, 5 µg/ml for Immunocytochemistry
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Applications:
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Western blotting, Immunocytochemistry
(heat-induced antigen-retrieval improves signal)
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Antigen Species Cross-Reactivity:
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human and bovine
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Storage:
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4°C
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Country of Origin:
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USA
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Product References
References for Complex IV subunit IV
There are no references currently posted for this product.
Images
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