MS405 Complex IV subunit II
monoclonal antibody


$295.00 (100 µg)

Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.


Brief Description & Uses

100 µg of monoclonal antibody reactive against the 26 kDa COX (Complex IV) subunit II. MS405 also labels COX II in immunocytochemical applications. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here. Variations in the synthesis of cytochrome c oxidase subunits from the two genomes (mitochondrial and nuclear) have been described as markers for several cancers including those of the prostate, and thyroid. Additionally this ratio has been reported to identify areas of the brain damaged in Alzheimer’s disease and also to relate to the overall regulation of mitochondrial biogenesis in exercise and diet. To examine this ratio in immunocytochemistry we offer two mAbs against mitochondrially encoded subunits I and II (MS404 and MS405, respectively) and mAbs against two nuclear encoded subunits IV and Va (MS408 and MS409, respectively). The latter two are retained in mitochondria exhibiting heterolpasmy or mosaicism of altered mtDNA transcription e.g. in anti-retroviral therapy. Therefore an immunocytochemical study using a combination of subunits I or II alongside IV or Va could reveal changes in the levels of mtDNA or mtDNA encoded proteins.


Specifications

Product: Complex IV subunit II
monoclonal antibody
Catalog #: MS405
Lot #:
Clone ID: 12C4F12
Host Species and Isotype: Mouse IgG2a, κ
Immunogen: Human Complex IV subunit II, from SDS-PAGE
Concentration: 1 mg/ml in HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 1.0 µg/ml for Western blotting, 5-10 µg/ml for Immunocytochemistry
Applications: Western blotting, Immunocytochemistry
(heat-induced antigen-retrieval improves signal)
Antigen Species Cross-Reactivity: human
Storage: 4°C
Country of Origin: USA



Product References

Genome Res. 2007 Aug;17:1319-1326.
"Direct linkage of mitochondrial genome variation to risk factors for type 2 diabetes in conplastic strains."

J. Physiol. 2006 Sept;575(3):901-11
"Short-term sprint interval versus traditional endurance training: similar initial adaptations in human skeletal muscle and exercise performance."

J. Clinical Endocrinology & Metabolism. 2006 April;91(4):1520 -1525
"Reduced Skeletal Muscle Uncoupling Protein-3 Content in Prediabetic Subjects and Type 2 Diabetic Patients: Restoration by Rosiglitazone Treatment."

Have you published research using this product?
Let us know and earn a $100.00 discount on your next purchase.



References for Complex IV subunit II

Nucleic Acids Res. 2006 Aug;34(13):e95
"Production of transmitochondrial cybrids containing naturally occurring pathogenic mtDNA variants."



Images






Downloadable Information
MS101 Specifications Western Blotting Protocol Immunocytochemistry Protocol Research Playbook


Additional Information
• Product Images
• Product References
• Protein References


Related Products
• Western Blotting Controls

• Secondary Antibodies
Home - About Us - Products - Support - Resources - Careers - Contact Us - Shopping Cart

Sales & Customer Support:
1-800-910-6486
[email protected]

Copyright © 2004-2008 MitoSciences Inc. All rights reserved.