MS404 Complex IV subunit I
monoclonal antibody

$295.00 (100 g)

Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.

Brief Description & Uses

100 g of monoclonal antibody reactive against the 57 kDa COX (Complex IV) subunit I. The apparent molecular weight is 35 kDa. MS404 also labels COX I in immunocytochemical applications. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here. Variations in the synthesis of cytochrome c oxidase subunits from the two genomes (mitochondrial and nuclear) have been described as markers for several cancers including those of the prostate, and thyroid. Additionally this ratio has been reported to identify areas of the brain damaged in Alzheimer’s disease and also to relate to the overall regulation of mitochondrial biogenesis in exercise and diet.

To examine this ratio in immunocytochemistry we offer two mAbs against mitochondrially encoded subunits I and II (MS404 and MS405, respectively) and mAbs against two nuclear encoded subunits IV and Va (MS408 and MS409, respectively). The latter two are retained in mitochondria exhibiting heterolpasmy or mosaicism of altered mtDNA transcription e.g. in anti-retroviral therapy. Therefore an immunocytochemical study using a combination of subunits I or II alongside IV or Va could reveal changes in the levels of mtDNA or mtDNA encoded proteins. When conducting immunocytochemistry it is critical to perform the antigen retrieval procedure (step C, page 6 of the protocol).


Product: Complex IV subunit I
monoclonal antibody
Catalog #: MS404
Lot #:
Clone ID: 1D6E1A8
Host Species and Isotype: Mouse IgG2a, κ
Immunogen: Human Complex IV subunit I, from SDS-PAGE
Concentration: 1 mg/ml in HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 1 g/ml for Western blotting, 5 g/ml for Immunocytochemistry
Applications: Western blotting, Immunocytochemistry
Antigen Species Cross-Reactivity: human, bovine, mouse, rat, Zebrafish, C. elegans, and oyster
Storage: 4C
Country of Origin: USA

Product References

J Clin Endocrin Metab. 2008 Jan 22; doi:10.1210/jc.2007-1701
"Activation of peroxisome proliferator activated receptor pathway stimulates the mitochondrial repiratory chain and can correct deficiencies in patients' cells lacking its components."

Plos Bio. 2007 Oct;5(10):e259.
"Relationship Between Mitochondrial Electron Transport Chain Dysfunction, Development, and Life Extension in Caenorhabditis elegans ."

PloS One 2007 Oct;2(10):e1066.
"Hsp90 Inhibition Decreases Mitochondrial Protein Turnover."

EMBO Journal 2007 Sept;26:4335-46.
"Coa1 links the Mss51 post-translational function to Cox1 cofactor insertion in cytochrome c oxidase assembly."

J. Bio. Chem. 2007 Aug;282(32):23613-21.
"Compartment-specific Phosphorylation of Phosducin in Rods Underlies Adaptation to Various Levels of Illumination."

Genome Res. 2007 Aug;17:1319-1326.
"Direct linkage of mitochondrial genome variation to risk factors for type 2 diabetes in conplastic strains."

J Biol Chem. 2007 Aug;doi:10.1074/jbc.M703528200
"Early developmental pathology due to cytochrome c oxidase deficiency is revealed by a new zebrafish model."

J Bio. Chem. 2007 June;282(24):17557-62.
"An Assembled Complex IV Maintains the Stability and Activity of Complex I in Mammalian Mitochondria."

Biochimica et Biophysica Acta 2007 May;1772:533-42.
"Yeast NDI1 improves oxidative phosphorylation capacity and increases protection against oxidative stress and cell death in cells carrying a Leber's hereditary optic neuropathy mutation ."

Neurosci. Letters 2007 Apr;416:160-4.
"Ethanol withdrawal posttranslationally decreases the activity of cytochrome c oxidase in an estrogen reversible manner."

Nature Gen. 2007 Mar;39:534-9.
"Mitochondrial aspartyl-tRNA synthetase deficiency causes leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation."

Hum Molec Genetics. 2007 Jan 8;16(4):431-44.
"Increased longevity and refractoriness to Ca2+ -dependent neurodegeneration in Surf1 knockout mice."

Invest. Ophthal. and Visual Sci. 2007;48:5091-100..
"Crucial Role of Apoptosis in the Resolution of Experimental Autoimmune Anterior Uveitis."

J Bioenerg Biomembr. 2006 Dec;38(5-6):283-91
"Cytochrome c oxidase subunit IV is essential for assembly and respiratory function of the enzyme complex."

Mol Biol Cell. 2006 Aug
"ATP-binding cassette transporters are required for efficient RNA interference in Caenorhabditis elegans."

J Biol Chem. 2006 Jul 6
"Human mitochondrial DNA nucleoids are linked to protein folding machinery and metabolic enzymes at the mitochondrial inner membrane."

Biochim Biophys Acta. 2006 Feb;1757(2):115-22
"Riboflavin enhances the assembly of mitochondrial cytochrome c oxidase in C. elegans NADH-ubiquinone oxidoreductase mutants."

J Clin Invest. 2005 Oct;115(10):2784-92.
"A molecular chaperone for mitochondrial complex I assembly is mutated in a progressive encephalopathy."

References for Complex IV subunit I


Complex IV subunit I Western Blotting Image

Image of the CcoI antibody (green) used to stain MELAS (myoclonus, epilepsy, lactic acidosis and stroke like episodes) where a mutation in a mitochondrially-encoded tRNA gene alters the biogenesis of Cco. A noted difference in staining can be seen between cells which have the CcoI subunit and those which either have little to none of the subunit present. The antibody was used at 7.5 ug/mL of anti-Cco subunit 1 mAb (MS404) in 10% goat serum, overnight at 4C, when testing fibroblasts or other adherent cells.

Downloadable Information
MS404 Specifications Western Blotting Protocol Immunocytochemistry Protocol Research Playbook

Additional Information
Product Images
Product References
Protein References

Related Products
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Secondary Antibodies

MS851 Isolation Kit for Rodent Tissue
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