MS402 MitoProfile® Complex IV Immunocapture Kit for Rodent
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250 µg Kit: $375.00
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500 µg Kit: $675.00
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750 µg Kit: $875.00
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Please contact us for volume discounts.
Brief Description & Uses
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked
to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or
~75 µg respectively of Complex IV from heart mitochondria. Isolated
mitochondria are available from several species for use as control
samples. To see the complete list of control samples available from
MitoSciences, click here.
The MitoProfile® Complex IV Immunocapture Kit for
Rodent allows isolation of the cytochrome c oxidase complex (E.C.
1.9.3.1) from small amounts of tissue. This facilitates subsequent
analysis of both assembly state and accrued post-translational
modifications of all 13 subunits of the enzyme. The immunoprecipitated
Complex IV shows cytochrome c oxidase activity while bound to the beads
and this activity is fully sensitive to cyanide. Uses for the Complex
IV immunocapture kit include but are not limited to examining
alterations of Complex IV subunits in inherited mitochondrial diseases,
Alzheimer’s disease, schizophrenia and cancer.
Specifications
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Product:
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MitoProfile® Complex IV Immunocapture Kit for Rodent
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Kit Components:
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250 µg of antibody crosslinked to protein G-agarose beads,
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Catalog #:
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MS402
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Lot #:
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Clone ID:
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DA5BC4
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Host Species and Isotype:
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Mouse IgG2a, κ
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Immunogen:
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Yeast native Complex IV
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Concentration:
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250 µg of capture antibody crosslinked to 25 µl matrix in 400 µl Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Suggested Working Concentration:
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10 µl matrix capture approximately 10 µg complex IV (COX) from 1 mg solubilized rat/mouse heart mitochondria.
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Applications:
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Immunoprecipitation
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Antigen Species Cross-Reactivity:
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rat and mouse
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Storage:
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4°C
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Country of Origin:
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USA
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Product References
There are no references currently posted for this product.
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References for Complex IV (cytochrome c oxidase)
There are no references currently posted for this product.
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