MS402 MitoProfile Complex IV Immunocapture Kit for Rodent


250 g Kit: $375.00
500 g Kit: $675.00
750 g Kit: $875.00
Please contact us for volume discounts.


Brief Description & Uses

250 g, 500 g or 750 g monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 g, ~50 g or ~75 g respectively of Complex IV from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The MitoProfile Complex IV Immunocapture Kit for Rodent allows isolation of the cytochrome c oxidase complex (E.C. 1.9.3.1) from small amounts of tissue. This facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the enzyme. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for the Complex IV immunocapture kit include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer’s disease, schizophrenia and cancer.


Specifications

Product: MitoProfile Complex IV Immunocapture Kit for Rodent

Kit Components:

250 g of antibody crosslinked to protein G-agarose beads,

Catalog #: MS402
Lot #:
Clone ID: DA5BC4
Host Species and Isotype: Mouse IgG2a, κ
Immunogen: Yeast native Complex IV
Concentration: 250 g of capture antibody crosslinked to 25 l matrix in 400 l Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 10 l matrix capture approximately 10 g complex IV (COX) from 1 mg solubilized rat/mouse heart mitochondria.
Applications: Immunoprecipitation
Antigen Species Cross-Reactivity: rat and mouse
Storage: 4C
Country of Origin: USA



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References for Complex IV (cytochrome c oxidase)

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Downloadable Information
MS402 Specifications Immunocapture Protocol Research Playbook


Additional Information
Product Images
Product References
Protein References


Related Products
Immunocapture Controls

Secondary Antibodies

Detergents

MS851 Isolation Kit for Rodent Tissue

MS401 Complex IV Immunocapture Kit
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