MS401 MitoProfile® Complex IV Immunocapture Kit
|
250 µg Kit: $375.00
|
|
|
500 µg Kit: $675.00
|
|
|
750 µg Kit: $875.00
|
|
Please contact us for volume discounts.
Brief Description & Uses
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked
to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or
~75 µg respectively of Complex IV from heart mitochondria. Isolated
mitochondria are available from several species for use as control
samples. To see the complete list of control samples available from
MitoSciences, click here.
The MitoProfile® Complex IV Immunocapture Kit allows
isolation of the cytochrome c oxidase complex (E.C. 1.9.3.1) from small
amounts of tissue. This facilitates subsequent analysis of both
assembly state and accrued post-translational modifications of all 13
subunits of the enzyme. The immunoprecipitated Complex IV shows
cytochrome c oxidase activity while bound to the beads and this
activity is fully sensitive to cyanide. Uses for the Complex IV
immunocapture kit include but are not limited to examining alterations
of Complex IV subunits in inherited mitochondrial diseases, Alzheimer’s
disease, schizophrenia and cancer.
Specifications
|
Product:
|
MitoProfile® Complex IV Immunocapture Kit
|
|
Kit Components:
|
250 µg of antibody crosslinked to protein G-agarose beads, BHM control.
|
|
Catalog #:
|
MS401
|
|
Lot #:
|
|
|
Clone ID:
|
31E91B82G9
|
|
Host Species and Isotype:
|
Mouse IgG1, κ
|
|
Immunogen:
|
Bovine Complex IV
|
|
Concentration:
|
250 µg of capture antibody crosslinked to 25 µl
matrix in 400 µl Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02%
azide as a preservative.
|
|
Purity:
|
Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
|
|
Suggested Working Concentration:
|
10 µl matrix capture approximately 10 µg complex IV (COX) from 1 mg solubilized bovine heart mitochondria.
|
|
Applications:
|
Immunoprecipitation
|
|
Antigen Species Cross-Reactivity:
|
human and bovine
|
|
Storage:
|
4°C
|
|
Country of Origin:
|
USA
|
Product References
References for Complex IV (cytochrome c oxidase)
There are no references currently posted for this product.
Images
|
CART
