MS301 MitoProfile®
Complex III Immunocapture Kit
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250
µg Kit: $375.00 |
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| 500 µg Kit: $675.00
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| 750 µg Kit: $875.00
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Please contact us for
volume discounts.
Brief Description & Uses
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked
to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or
~75 µg respectively of Complex III from heart mitochondria. Isolated
mitochondria are available from several species for use as control
samples. To see the complete list of control samples available from
MitoSciences, click
here.
The MitoProfile® Complex III Immunocapture Kit
allows isolation of the ubiquinol-cytochrome c oxidoreductase complex
(E.C. 1.10.2.2) from small amounts of tissue. This facilitates
subsequent analysis of assembly state, activity and the extent of post
translational modifications including oxidative damage that occur with
aging. Uses for the Complex III immunocapture kit include research on
genetic mitochondrial disease, where complex III may have an additional
role in the stabilization of complex I.
Specifications
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Product:
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MitoProfile® Complex III Immunocapture Kit
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Kit Components:
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250 µg of antibody crosslinked to
protein G-agarose beads, BHM control.
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Catalog #:
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MS301
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Lot #:
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Clone ID:
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11A51H12
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Host Species and Isotype:
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Mouse IgG1, κ
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Immunogen:
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Bovine Heart Complex III
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Concentration:
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250 µg of capture antibody
crosslinked to 25 µl matrix in 400 µl HEPES-Buffered Saline (HBS) with
0.02% azide as a preservative.
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Purity:
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Near homogeneity as judged by SDS-PAGE.
The antibodies were produced in vitro using
hybridomas grown in serum-free medium, and then purified by biochemical
fractionation.
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Suggested Working Concentration:
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10 µl matrix
capture approximately 10 µg complex III from 1 mg solubilized bovine
heart mitochondria.
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Applications:
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Immunoprecipitation
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Antigen Species Cross-Reactivity:
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human, bovine,
mouse, and rat
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Storage:
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4°C
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Country of Origin:
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USA
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Product
References
References
for Complex III (Ubiquinol cytochrome c oxidoreductase)
There are no references currently posted for this product.
Images
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CART
