MS301 MitoProfile Complex III Immunocapture Kit


250 g Kit: $375.00
500 g Kit: $675.00
750 g Kit: $875.00
Please contact us for volume discounts.


Brief Description & Uses

250 g, 500 g or 750 g monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 g, ~50 g or ~75 g respectively of Complex III from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The MitoProfile Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex (E.C. 1.10.2.2) from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex III immunocapture kit include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.


Specifications

Product: MitoProfile Complex III Immunocapture Kit

Kit Components:

250 g of antibody crosslinked to protein G-agarose beads, BHM control.

Catalog #: MS301
Lot #:
Clone ID: 11A51H12
Host Species and Isotype: Mouse IgG1, κ
Immunogen: Bovine Heart Complex III
Concentration: 250 g of capture antibody crosslinked to 25 l matrix in 400 l HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 10 l matrix capture approximately 10 g complex III from 1 mg solubilized bovine heart mitochondria.
Applications: Immunoprecipitation
Antigen Species Cross-Reactivity: human, bovine, mouse, and rat
Storage: 4C
Country of Origin: USA



Product References




References for Complex III (Ubiquinol cytochrome c oxidoreductase)

There are no references currently posted for this product.




Images

Complex III immunoprecipitation results


Downloadable Information
MS101 Specifications Immunocapture Protocol


Additional Information
Product Images
Product References
Protein References


Related Products
Immunocapture Controls

Secondary Antibodies

Detergents

MS851 Isolation Kit for Rodent Tissue

MS301c Complex III Immunocapture mAb
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