MS201c Complex II Immunocapture
monoclonal antibody


$295.00 (100 µg)

Please contact us for volume discounts.


Brief Description & Uses

100 µg monoclonal antibody which can immunocapture ~5 µg of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The Complex II immunocapture kit allows isolation of the succinate dehydrogenase complex (E.C. 1.3.5.1) from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.


Specifications

Product: Complex II Immunocapture
monoclonal antibody
Catalog #: MS201c
Lot #:
Clone ID: 4H12BG12AG2
Host Species and Isotype: Mouse IgG1, κ
Immunogen: Bovine Heart Complex II, with traces of Complex III
Concentration: 1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 100 µg mAb can capture at least 5 µg complex II from 1 mg solubilized bovine heart mitochondria.
Applications: Immunoprecipitation
Antigen Species Cross-Reactivity: human, bovine and mouse
Storage: 4°C
Country of Origin: USA



Product References

Toxicology In Vitro. 2007 Aug;21(5):902-11.
"Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays."

Biochim Biophys Acta. 2006 Feb;1762(2):213-22.
"Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria."

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References for Complex II (Succinate ubiquinone oxidoreductase)

There are no references currently posted for this product.




Images



Downloadable Information
MS101 Specifications Immunocapture Protocol


Additional Information
• Product Images
• Product References
• Protein References


Related Products
• Immunocapture Controls

• Secondary Antibodies

• Detergents

• MS851 Isolation Kit for Rodent Tissue

• MS201 Complex II Immunocapture Kit
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