MS201 MitoProfile® Complex II Immunocapture Kit
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250 µg Kit: $375.00
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500 µg Kit: $675.00
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750 µg Kit: $875.00
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Please contact us for volume discounts.
Brief Description & Uses
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked
to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or
~39 µg respectively of Complex II from heart mitochondria. Isolated
mitochondria are available from several species for use as control
samples. To see the complete list of control samples available from
MitoSciences, click here.
The MitoProfile® Complex II Immunocapture Kit allows
isolation of the succinate dehydrogenase complex (E.C. 1.3.5.1) from
small amounts of tissue. This facilitates subsequent analysis of
assembly state, activity and the extent of post translational
modifications including oxidative damage that occur with aging. Uses
for the Complex II immunocapture kit include research on aging genetic
mitochondrial disease, and various cancers due to mutations in this
enzyme complex such as paragangliomas and phaeo-chromocytomas.
Specifications
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Product:
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MitoProfile® Complex II Immunocapture Kit
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Kit Components:
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250 µg of antibody crosslinked to protein G-agarose beads, BHM control.
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Catalog #:
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MS201
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Lot #:
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Clone ID:
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4H12BG12AG2
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Host Species and Isotype:
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Mouse IgG1, κ
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Immunogen:
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Bovine Heart Complex II, with traces of Complex III
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Concentration:
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250 µg of capture antibody crosslinked to 25 µl matrix in 400 µl HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Suggested Working Concentration:
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10 µl matrix capture approximately 5 µg complex II from 1 mg solubilized bovine heart mitochondria.
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Applications:
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Immunoprecipitation
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Antigen Species Cross-Reactivity:
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human, bovine, mouse and rat
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Storage:
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4°C
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Country of Origin:
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USA
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Product References
References for Complex II (Succinate ubiquinone oxidoreductase)
There are no references currently posted for this product.
Images
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