MS133 MitoProfile® Dipstick Assay Kit
for Rodent Complex I Quantity
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30 Dipstick Kit: $325.00
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60 Dipstick Kit: $545.00
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Please contact us for volume discounts.
Brief Description & Uses
Contains 30 or 60 dipsticks and necessary components to quantify the
levels of the fully assembled Complex I enzyme complex (NADH ubiquinone
oxidoreductase) from mouse and rat samples. The kit includes sufficient
materials to generate a standard curve and evaluate several unknown
samples.
For a detailed overview of MitoSciences Dipstick Assay Technology please click here.
Immunochromatographic Reader
While visual measurements and scanning on a flatbed scanner can both be
used to evaluate our dipsticks, the most effective quantitative results
are derived when using an immunochromatographic reader that has been
specifically designed to read conjugated gold lateral flow devices.
The reader that we recommend is the Hamamatsu ICA-1000,
which is a simple PC-based system for measuring and analyzing our
dipstick products. We have an agreement in place with Hamamatsu that
allows us to offer this reader directly to our customers. To learn more
about this reader, or to order one, please click here to visit the product page.
Specifications
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Product:
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MitoProfile® Dipstick Assay Kit for Rodent Complex I Quantity
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Catalog #:
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MS133
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Kit Components:
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Dipsticks, dried Complex I gold-conjugated antibody, and buffers.
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mAb Clone ID:
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18G12BC2 (dipsticks)
6E1BH7 (dried gold-conjugated Complex I subunit GRIM-19 monoclonal antibody)
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Applications:
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Quantitative analysis of Complex I
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Antigen Species Cross-Reactivity:
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Mouse, Rat
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Storage:
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Store dipsticks and gold-conjugated secondary antibody at room
temperature out of direct sunlight in their provided containers. Store
buffers at 4°C.
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Country of Origin:
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USA
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Product References
References for Complex I Dipstick Assays
There are no references currently posted for this product.
Images
The above results show the clear visual difference between a sample
with normal levels of Complex I enzyme complex compared to patients
with defects in Complex I assembly (patient 1 and 2) or cells depleted
of mitochondrial proteins (rho0 cells). Also, we can see that the
dipsticks are specific since they detect no alteration in Complex I
assembly from a Complex IV patient. The dipstick results are both
quantitative and highly reproducible (intra-assay error less than 10%).
The developed dipsticks can be analyzed with a simple flat-be scanner
and common imaging software or, for sensitive and reproducible results,
a dipstick reader can be utilized (our suggested reader: Hamamatsu ICA-1000).
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CART
