MS107 Complex I subunit NDUFB4
monoclonal antibody


$295.00 (100 µg)

Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.


Brief Description & Uses

100 µg of monoclonal antibody reactive against the 15 kDa Complex I (NADH dehydrogenase) subunit NDUFB4. MS107 also labels in immunocytochemical applications. Useful in studying Complex I assembly. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

Previously, based on MW and isoelectric point on gels MS107 was tentatively identified as likely reacting against subunit NDUFA6; mass spectometry now confirms identification of it as subunit NDUFB4.


Specifications

Product: Complex I subunit NDUFB4
monoclonal antibody
Catalog #: MS107
Lot #:
Clone ID: 17G3D9E12
Host Species and Isotype: Mouse IgG1, κ
Immunogen: Bovine Complex I
Concentration: 1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 0.5 µg/ml for Western blotting, 5-15 µg/ml for Immunocytochemistry
Applications: Western blotting, Immunocytochemistry
(heat-induced antigen-retrieval improves signal)
Antigen Species Cross-Reactivity: human, bovine and rat (weak)
Storage: 4°C
Country of Origin: USA



Product References

J Bio. Chem.. 2007;282(10):7582-90.
"Identification of Mitochondrial Complex I Assembly Intermediates by Tracing Tagged NDUFS3 Demonstrates the Entry Point of Mitochondrial Subunits."

J Neurosci. 2006 May 10;26(19):5256-64.
"Parkinson's disease brain mitochondrial complex I has oxidatively damaged subunits and is functionally impaired and misassembled."

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References for Complex I subunit NDUFB4

There are no references currently posted for this product.




Images

Complex I subunit NDUFB4 Western Blotting Image


Complex I subunit NDUFB4 Immunocytochemistry Image


Downloadable Information
MS107 Specifications Western Blotting Protocol Immunocytochemistry Protocol


Additional Information
• Product Images
• Product References
• Protein References


Related Products
• Western Blotting Controls

• Secondary Antibodies

• MS851 Isolation Kit for Rodent Tissue
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