MS103 Complex I subunit GRIM-19
monoclonal antibody


$295.00 (100 µg)

Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.


Brief Description & Uses

100 µg of monoclonal antibody reactive against the 19 kDa Complex I (NADH dehydrogenase) subunit GRIM-19. MS103 also labels GRIM-19 in immunocytochemical applications. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

GRIM-19 (Gene associated with Retinoid IFN induced Mortality) was first described as an apoptotic factor located in the cytosol and nucleus of cells undergoing programmed cell death. Recently this same protein was shown to be a subunit of Complex I in bovine and human mitochondria.

The anti GRIM-19 mAb reacts specifically in Western blots and immunocytochemistry, and shows cross reactivity with human, bovine, mouse and rat tissues. This mAb can be used to evaluate the interaction of GRIM-19 with Complex I in vivo or after immunocapture as a result of various cell treatments and may also be useful in other kinds of studies of apoptosis.


Specifications

Product: Complex I subunit GRIM-19
monoclonal antibody
Catalog #: MS103
Lot #:
Clone ID: 6E1BH7
Host Species and Isotype: Mouse IgG2b, κ
Immunogen: Full-length recombinant human GRIM-19
Concentration: 1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 1 µg/ml for Western blotting, 1 µg/ml for Immunocytochemistry
Applications: Western blotting, Immunocytochemistry
(heat-induced antigen-retrieval improves signal)
Antigen Species Cross-Reactivity: human, bovine, mouse and rat
Storage: 4°C
Country of Origin: USA



Product References

J Interferon and Cytokine Research 2007;27(5):383-92.
"The IFN-β and Retinoic Acid-Induced Cell Death Regulator GRIM-19 is Upregulated During Focal Cerebral Ischemia."

Science. 2007 June;316(5829):1345-8.
"Complex I binding by a virally encoded RNA regulates mitochondria-induced cell death."

Mol Cell Biol. 2006 Feb;26(3):1077-86.
"Nuclear suppression of mitochondrial defects in cells without the ND6 subunit."

J Clin Invest. 2005 Oct;115(10):2784-92.
"A molecular chaperone for mitochondrial complex I assembly is mutated in a progressive encephalopathy."

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References for Complex I subunit GRIM-19

MitoNews, Volume 01, Issue 08
"AIF and a caspase-independent pathway of apoptosis"

MitoNews, Volume 01, Issue 07
"Grim-19 mutations as a cause of Hurthie cell (thyroid) tumors"




Images

GRIM-19 Western Blotting Image


GRIM-19 Immunocytochemistry Image


Downloadable Information
MS101 Specifications Western Blotting Protocol Immunocytochemistry Protocol


Additional Information
• Product Images
• Product References
• Protein References


Related Products
• Western Blotting Controls

• Secondary Antibodies

• MS851 Isolation Kit for Rodent Tissue
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