MS101 MitoProfile® Complex I Immunocapture Kit
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250 µg Kit: $375.00
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500 µg Kit: $675.00
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750 µg Kit: $875.00
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Please contact us for volume discounts.
Brief Description & Uses
250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked
to protein G-agarose beads which can immunocapture ~60 µg, ~120 µg or
~180 µg respectively of Complex I (NADH dehydrogenase) from heart
mitochondria. Isolated mitochondria are available from several species
for use as control samples. To see the complete list of control samples
available from MitoSciences, click here.
The MitoProfile® Complex I Immunocapture Kit allows
isolation of the NADH ubiquinone oxidoreductase complex (E.C. 1.6.5.3)
from small amounts of tissue. This facilitates subsequent analysis of
assembly state and activity. Thus the enzyme retains NADH-ferricyanide,
NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after
isolation, and with added lipids it also shows significant rotenone
sensitivity.
Finally, the extent of post translational modifications including
oxidative damage can be readily analyzed by proteomic approaches or
antibody detection of these modifications. Uses for the Complex I
immunocapture kit include but are not limited to examining alterations
of Complex I subunits in inherited mitochondrial diseases, Parkinson's
disease, Alzheimer's disease, ALS, schizophrenia, and aging.
Specifications
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Product:
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MitoProfile® Complex I Immunocapture Kit
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Kit Components:
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250 µg of antibody crosslinked to protein G-agarose beads, BHM control.
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Catalog #:
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MS101
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Lot #:
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Clone ID:
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18G12
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Host Species and Isotype:
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Mouse IgG2b, κ
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Immunogen:
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Bovine Heart Complex I
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Concentration:
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250 µg of capture antibody crosslinked to 25 µl matrix in 400 µl HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Purity:
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Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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Suggested Working Concentration:
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10 µl matrix capture approximately 25 µg complex I from 1 mg solubilized bovine heart mitochondria.
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Applications:
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Immunoprecipitation
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Antigen Species Cross-Reactivity:
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human, bovine, rat and mouse
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Storage:
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4°C
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Country of Origin:
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USA
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Product References
References for Complex I (NADH ubiquinone oxidoreductase)
Images
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CART
