The Mitochondrial trifunctional protein (TFP) subunit alpha and beta (HADHA / HADHB) FlexPlex™ Module is used to determine the quantity of TFP (mitochondrial trifunctional protein, subunit alpha / subunit beta) enzyme in a sample. A functional fully assembled TFP is immunocaptured within the wells by an antibody specific to the alpha subunit. The quantity of protein is measured by adding a second TFP specific antibody that is then labeled with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue. The rate of color development is proportional to the amount of protein captured in the well and can be monitored at 600 nm. Alternatively, the assay can be terminated, at a user defined time, by the addition of 1N HCl (not supplied) and the assay performed as an end point measurement at 450 nm.
Click here to learn more about Mitochondrial trifunctional protein (TFP) subunit alpha and beta (HADHA / HADHB).